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Heat Stress and Agonists of Muscarinic Cholinergic Receptors Modulate Sensitivity of Nicotinic Cholinergic Receptors in Soil Nematode Caenorhabditis elegans

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We studied the effect of moderate heat stress (30oC) and muscarinic cholinergic receptor agonists arecoline and pilocarpine on sensitivity of the behavior of the nematode Caenorhabditis elegans of N2 line to the action of the agonist of nicotinic cholinergic receptor agonist levamisole. Heat stress and muscarinic cholinergic receptor agonists increased the sensitivity of swimming induced by mechanical stimulation to levamisole (32-64 μM), which manifested in dyscoordination of locomotor muscles during swimming and complete loss of ability to swim. Combined exposure to heat stress and muscarinic cholinergic receptor agonists revealed their synergism in the influence on sensitivity of swimming behavior to levamisole: heating to 30oC potentiated the effect of arecoline and arecoline potentiated the effect of heat stress. It is assumed, that the effect of heat stress on the sensitivity of nicotinic receptors is mediated by its effect on muscarinic receptors.

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References

  1. Albuquerque EX, Pereira EF, Alkondon M, Rogers SW. Mammalian nicotinic acetylcholine receptors: from structure to function. Physiol. Rev. 2009;89(1):73-120.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Anderson GL, Cole RD, Williams PL. Assessing behavioral toxicity with Caenorhabditis elegans. Environ. Toxicol. Chem. 2004; 23(5):1235-1240.

    Article  CAS  PubMed  Google Scholar 

  3. Chan JP, Staab TA, Wang H, Mazzasette C, Butte Z, Sieburth D. Extrasynaptic muscarinic acetylcholine receptors on neuronal cell bodies regulate presynaptic function in Caenorhabditis elegans. J. Neurosci. 2013;33(35):14,146-14,159.

    Article  CAS  Google Scholar 

  4. Dittman JS, Kaplan JM. Behavioral impact of neurotransmitter- activated G-protein-coupled receptors: muscarinic and GABAB receptors regulate Caenorhabditis elegans locomotion. J. Neurosci. 2008;28(28):7104-7112.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Gainutdinov MK, Belova EB, Kalinnikova TB, Kolsanova RR, Shagidullin RR. Sensitization of nicotinic acetylcholine receptors in soil nematode Caenorhabditis elegans through activation of muscarinic receptors by arecoline. J. Evol. Biochem. Physiol. 2015;51(4):349-351.

    Article  Google Scholar 

  6. Ghosh R, Mohammadi A, Kruglyak L, Ryu WS. Multiparameter behavioral profiling reveals distinct thermal response regimes in Caenorhabditis elegans. BMC Biol. 2012;10:85. doi:https://doi.org/10.1186/1741-7007-10-85.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Glauser DA. How and why Caenorhabditis elegans uses distinct escape and avoidance regimes to minimize exposure to noxious heat. Worm. 2013;2:e27285. doi:https://doi.org/10.4161/worm.27285.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Jospin M, Qi YB, Stawicki TM, Boulin T, Schuske KR, Horvitz HR, Bessereau JL, Jorgensen EM, Jin Y. A neuronal acetylcholine receptor regulates the balance of muscle excitation and inhibition in Caenorhabditis elegans. PLoS Biol. 2009;7(12):e1000265. doi:https://doi.org/10.1371/journal.pbio.1000265.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Kalinnikova TB, Kolsanova RR, Belova EB, Shagidullin RR, Gainutdinov MK. Opposite effects of moderate heat stress and hyperthermia on cholinergic system of soil nematodes Caenorhabditis elegans and Caenorhabditis briggsae. J. Therm. Biol. 2016;62(Pt A):37-49.

    Article  CAS  PubMed  Google Scholar 

  10. Pereira L, Kratsios P, Serrano-Saiz E, Sheftel H, Mayo AE, Hall DH, White JG, LeBoeuf B, Garcia LR, Alon U, Hobert O. A cellular and regulatory map of the cholinergic nervous system of C. elegans. eLife. 2015;4. pii: e12432. doi:https://doi.org/10.7554/eLife.12432.

  11. Satelle DB. Invertebrate nicotinic acetylcholine receptors — targets for chemicals and drugs important in agriculture, veterinary medicine and human health. J. Pestic. Sci. 2009;34:233-240. doi:https://doi.org/10.1584/jpestics.r09-02.

    Article  Google Scholar 

  12. Steger KA, Avery L. The GAR-3 muscarinic receptor cooperates with calcium signals to regulate muscle contraction in the Caenorhabditis elegans pharynx. Genetics. 2004;167(2):633-643.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Tissenbaum HA, Hawdon J, Perregaux M, Hotez P, Guarente L, Ruvkun G. A common muscarinic pathway for diapause recovery in the distantly related nematode species Caenorhabditis elegans and Ancylostoma caninum. Proc. Natl Acad. Sci. USA. 2000;97(1):460-465.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Institute of Problems of Ecology and Subsoil Use, Academy of Sciences of the Republic of Tatarstan, Kazan, Tatarstan Republic, Russia

    T. B. Kalinnikova, A. F. Yakhina, A. V. Egorova, R. R. Shagidullin & M. H. Gainutdinov

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  1. T. B. Kalinnikova
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  2. A. F. Yakhina
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  3. A. V. Egorova
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  4. R. R. Shagidullin
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  5. M. H. Gainutdinov
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Correspondence to T. B. Kalinnikova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 8, pp. 169-172, August, 2017

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Kalinnikova, T.B., Yakhina, A.F., Egorova, A.V. et al. Heat Stress and Agonists of Muscarinic Cholinergic Receptors Modulate Sensitivity of Nicotinic Cholinergic Receptors in Soil Nematode Caenorhabditis elegans . Bull Exp Biol Med 164, 144–147 (2017). https://doi.org/10.1007/s10517-017-3944-2

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  • DOI: https://doi.org/10.1007/s10517-017-3944-2

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