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Modeling of Lymphogenous and Hematogenous Metastasizing from Murine В16 Melanoma in Rats

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Bulletin of Experimental Biology and Medicine Aims and scope

We developed a model of experimental melanoma. Intralienal xenogeneic transplantation of a suspension of melanoma cells B16 in physiological saline (0.1 ml; 1:10) was conducted to outbred male rats. In 6 months, histologically confirmed melanoma B16 in the spleen and its metastases in the liver, intestine, pancreas, adrenal glands, and lungs (hematogenous metastasis), as well as in the thymus and lymph nodes (lymphogenous metastasis) were revealed in rats. The proposed rat model of melanoma B16 metastasizes by the hematogenous and lymphogenous pathways, develops over 6 months, and allows receiving sufficient volume of material for analysis.

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Authors and Affiliations

  1. Rostov Research Institute of Oncology, Ministry of Health of the Russian Federation, Rostov-on-Don, Russia

    O. I. Kit, I. V. Kaplieva, E. M. Frantsiyants & L. K. Trepitaki

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  1. O. I. Kit
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  2. I. V. Kaplieva
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  3. E. M. Frantsiyants
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  4. L. K. Trepitaki
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Corresponding author

Correspondence to I. V. Kaplieva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 6, pp. 759-762, June, 2017

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Kit, O.I., Kaplieva, I.V., Frantsiyants, E.M. et al. Modeling of Lymphogenous and Hematogenous Metastasizing from Murine В16 Melanoma in Rats. Bull Exp Biol Med 163, 793–795 (2017). https://doi.org/10.1007/s10517-017-3905-9

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  • DOI: https://doi.org/10.1007/s10517-017-3905-9

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