External magnetic field is characterized by low toxicity and existence of magnetic properties, which contributes to an interest in the development of products from ferromagnetic nanoparticles (FNP) for antitumor therapy. Previously we synthesized a conjugate of ferromagnetic magnetite nanoparticles and viscumin (mistletoe lectin I, MLI), which exhibits the antitumor activity. Studying the pharmacological properties of this conjugate (FNP-MLI) was directed to the evaluation of FNP-MLI elimination after intratumor injection in mice. The elimination rate of FNP-MLI was much lower than that of native plant MLI. The presence of FNP-MLI was not accompanied by undesired changes in the tumor tissue. The use of a FNP-MLI conjugate allowed us to prolong the time of MLI presence in tissues without increasing the dose of exogenous lectin. These features contribute to the prolongation of an immunomodulatory effect of MLI.
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Maltseva DV, Gerasimov VM, Sakharov DA, Shkurnikov MYu. Target Cell Glycosylation Determines the Biodistribution of Viscumin, a Plant Lectin. Bull. Exp. Biol. Med. 2017;163(4):481-485.
Maltseva DV, Krainova NA, Khaustova NA, Nikulin SV, Tonevitskaya SA, Poloznikov AA. Biodistribution of Viscumin after Subcutaneous Injection to Mice and In Vitro Modeling of Endoplasmic Reticulum Stress. Bull. Exp. Biol. Med. 2017;163(4):448-453.
Khutornenko AA, Gerasimov VM, Sakharov DA. Preparation of Viscumin-Ferromagnetic Particles Conjugate and Study of Its Internalization by Human Glioblastoma A172 Cells. Bull. Exp. Biol. Med. 2015;160(6):823-826.
Agapov II, Tonevitsky AG, Maluchenko NV, Moisenovich MM, Bulah YS, Kirpichnikov MP. Mistletoe lectin A-chain unfolds during the intracellular transport. FEBS Lett. 1999;464(1-2):63-66.
Agapov II, Tonevitsky AG, Moysenovich MM, Maluchenko NV, Weyhenmeyer R, Kirpichnikov MP. Mistletoe lectin dissociates into catalytic and binding subunits before translocation across the membrane to the cytoplasm. FEBS Lett. 1999;452(3):211-214.
Grootjans J, Kaser A, Kaufman RJ, Blumberg RS. The unfolded protein response in immunity and inflammation. Nat. Rev. Immunol. 2016;16(18):469-484.
Manevich EM, Tonevitsky AG, Bergelson LD. The binding of the B-chain of ricin to Burkitt lymphoma cells. FEBS Lett. 1986;194(2):313-316.
Mistletoe: From Mythology to Evidence-Based Medicine. Zänker KS, Kaveri SV, Chan SHH, eds. Basel, 2015.
Moisenovic M, Tonevitsky A, Agapov I, Niwa H, Schewe H, Bereiter-Hahn J. Differences in endocytosis and intracellular sorting of ricin and viscumin in 3T3 cells. Eur. J. Cell Biol. 2002;81(10):529-538.
Moisenovich M, Tonevitsky A, Maljuchenko N, Kozlovskaya N, Agapov I, Volknandt W, Bereiter-Hahn J. Endosomal ricin transport: involvement of Rab4-and Rab5-positive compartments. Histochem. Cell Biol. 2004;121(6):429-439.
Schumacher U, Pfüller U. From Berlin and Witten to Southampton and Hamburg: 25 Years of Mistletoe Research Cooperation. Mistletoe: From Mythology to Evidence-Based Medicine. Zänker KS, Kaveri SV, Chan SHH, eds. Basel, 2015. Vol. 4. P. 39-47.
Sweeney EC, Tonevitsky AG, Palmer RA, Niwa H, Pfueller U, Eck J, Lentzen H, Agapov II, Kirpichnikov MP. Mistletoe lectin I forms a double trefoil structure. FEBS Lett. 1998;431(3):367-370.
Tonevitsky AG, Agapov II, Shamshiev AT, Temyakov DE, Pohl P, Kirpichnikov MP. Immunotoxins containing A-chain of mistletoe lectin I are more active than immunotoxins with ricin A-chain. FEBS Lett. 1996;392(2):166-168.
Tonevitsky AG, Agapov I, Temiakov D, Moisenovich M, Maluchenko N, Solopova O, Würzner G, Pfueller U. Study of heterogeneity of lectins in mistletoe preparations by monoclonal antibodies to their A-subunits. Arzneimittelforschung. 1999;49(11):970-975.
Tonevitsky AG, Marx U, Agapov I, Moisenovich M. Detection of isolated mistletoe lectin chains in plant extracts. Arzneimittelforschung. 2002;52(1):67-71.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 6, pp. 710-713, June, 2017
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Maltseva, D.V., Nikulin, S.V., Sergeeva, N.S. et al. Elimination of a Viscumin-Ferromagnetic Nanoparticles Conjugate from the Tumor Nodule in Mice. Bull Exp Biol Med 163, 745–748 (2017). https://doi.org/10.1007/s10517-017-3894-8
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DOI: https://doi.org/10.1007/s10517-017-3894-8