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Metagenomic Analysis of Gingival Sulcus Microbiota and Pathogenesis of Periodontitis Associated with Type 2 Diabetes Mellitus

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Biofilm of the gingival sulcus from 22 patients with type 2 diabetes mellitus and periodontitis, 30 patients with periodontitis not complicated by diabetes mellitus (reference group), and 22 healthy volunteers without signs of gingival disease (control group) was studied by quantitative PCR. Quantitative analysis for the content of P. gingivalis, T. forsythia, A. ctinomycetemcomitans, T. denticola, P. intermedia, F. nucleatum/periodonticum, and P. endodontalis in the dental plaque was performed with a Dentoscreen kit. The presence of other bacterial groups was verified by metagenomic sequencing of the 16S rRNA gene to evaluate some specific features of the etiological factor for periodontitis in type 2 diabetes mellitus. Specimens of the Porphiromonadaceae and Fusobacteriaceae families were characterized by an extremely high incidence in combined pathology. The amount of Sphingobacteriaceae bacteria in the biofilm was shown to decrease significantly during periodontitis. Metagenomic analysis confirmed the pathogenic role of microbiota in combined pathology, as well as the hypothesis on a possible influence of periodontitis on the course and development of type 2 diabetes mellitus.

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Authors and Affiliations

  1. A. I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of the Russian Federation, Moscow, Russia

    E. A. Babaev, I. P. Balmasova, A. M. Mkrtumyan, V. N. Tsarev & S. D. Arutyunov

  2. Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia

    S. N. Kostryukova, E. S. Vakhitova & E. N. Il’ina

  3. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    A. G. Gabibov

Authors
  1. E. A. Babaev
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  2. I. P. Balmasova
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  3. A. M. Mkrtumyan
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  4. S. N. Kostryukova
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  5. E. S. Vakhitova
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  6. E. N. Il’ina
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  7. V. N. Tsarev
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  8. A. G. Gabibov
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  9. S. D. Arutyunov
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Corresponding author

Correspondence to E. A. Babaev.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 6, pp. 682-686, June, 2017

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Babaev, E.A., Balmasova, I.P., Mkrtumyan, A.M. et al. Metagenomic Analysis of Gingival Sulcus Microbiota and Pathogenesis of Periodontitis Associated with Type 2 Diabetes Mellitus. Bull Exp Biol Med 163, 718–721 (2017). https://doi.org/10.1007/s10517-017-3888-6

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  • DOI: https://doi.org/10.1007/s10517-017-3888-6

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