Effect of Selank on Functional State of Rat Hepatocytes under Conditions of Restraint Stress

We studied the effect of Selank administered intraperitoneally in doses of 100, 300, and 1000 μg/kg to male Wistar rats 15 min prior to restraint stress on the content of aminotransferases and total protein concentration in blood serum and intensity of free radical oxidation in the liver. Under conditions of acute restraint stress, Selank in doses of 100 and 300 μg/kg decreased catalase and superoxide dismutase activities and malondialdehyde concentration and increased total antioxidant activity in the liver homogenate. Administration of Selank in a dose of 1000 μg/kg reduced the content of aminotransferases in blood serum, decreased superoxide dismutase activity in the liver, and increased total antioxidant activity. Under conditions of chronic stress, Selank in all doses produced similar effects: reduced superoxide dismutase activity and malondialdehyde concentration in the liver tissue and AST activity in the serum. The other parameters remained unchanged.

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References

  1. 1.

    Kozlovskaya MM, Sarkisova KYu, Kozlovsky II. The effect of heptapeptide Selank on behavioral depression in high and low anxious Balb/C and C57Bl/6 mice and WAG/Rij rats with inherited depressiveness of behavior. Psikhofarmakol. Biol. Narkol. 2005;5(3):989-996. Russian.

    Google Scholar 

  2. 2.

    Narkevich VB, Klodt PM, Kudrin VS, Maysky AI, Rayevsky KS. The effects of heptapeptide Selank on the excitatory and inhibitory neurotransmitter amino acids content in brain structures of Wistar rats. Psikhofarmakol. Biol. Narkol. 2007;7(2):1563-1567. Russian.

    Google Scholar 

  3. 3.

    Pavlov TS, Samonina GE, Bakaeva ZV, Zolotarev YA, Guseva AA. Selank and its metabolites maintain homeostasis in the gastric mucosa. Bull. Exp. Biol. Med. 2007;143(1):51-53.

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Khnuchenko LK, Sapronov NS. Stress and its role in pathological processes. Obzory po Klin. Farmakol. Lek. Ter. 2003;2(3):2-15. Russian.

    Google Scholar 

  5. 5.

    Bandegi AR, Rashidy-Pour A, Vafaei AA, Ghadrdoost B. Protective effects of Crocus Sativus L. extract and Crocin against chronic-stress induced oxidative damage of brain, liver and kidneys in rats. Adv. Pharm. Bull. 2014;4(Suppl 2):493-499.

    PubMed  PubMed Central  Google Scholar 

  6. 6.

    Chida Y, Sudo N, Kubo C. Does stress exacerbate liver diseases? J. Gastroenterol. Hepatol. 2006;21(1, Pt 2):202-208.

    CAS  Article  PubMed  Google Scholar 

  7. 7.

    Chida Y, Sudo N, Sonoda J, Sogawa H, Kubo C. Electric foot shock stress-induced exacerbation of alpha-galactosylceramide-triggered apoptosis in mouse liver. Hepatology. 2004;39(4):1131-1140.

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    Djordjevic J, Djordjevic A, Adzic M, Niciforovic A, Radojcic M.B. Chronic stress differentially affects antioxidant enzymes and modifies the acute stress response in liver of Wistar rats. Physiol. Res. 2010;59(5):729-736.

    CAS  PubMed  Google Scholar 

  9. 9.

    Liu YZ, Chen JK, Zhang Y, Wang X, Qu S, Jiang CL. Chronic stress induces steatohepatitis while decreases visceral fat mass in mice. BMC Gastroenterol. 2014;14:106. doi: https://doi.org/10.1186/1471-230X-14-106.

    Article  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Matés JM, Sánchez-Jiménez F. Antioxidant enzymes and their implications in pathophysiologic processes. Front. Biosci.1999;4:D339-D345.

    Article  PubMed  Google Scholar 

  11. 11.

    McIntosh LJ, Hong KE, Sapolsky RM. Glucocorticoids may alter antioxidant enzyme capacity in the brain: baseline studies. Brain Res. 1998;791(1-2):209-214.

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    McKay LI, Cidlowski JA. Molecular control of immune/inflammatory responses: interactions between nuclear factorkappa B and steroid receptor-signaling pathways. Endocr. Rev. 1999;20(4):435-459.

    CAS  PubMed  Google Scholar 

  13. 13.

    Miller GE, Chen E, Sze J, Marin T, Arevalo JM, Doll R, Ma R, Cole SW. A functional genomic fingerprint of chronic stress in humans: blunted glucocorticoid and increased NF-kappaB signaling. Biol. Psychiatry. 2008;64(4):266-272.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Sahin E, Gümüşlü S. Stress-dependent induction of protein oxidation, lipid peroxidation and anti-oxidants in peripheral tissues of rats: comparison of three stress models (immobilization, cold and immobilization-cold). Clin. Exp. Pharmacol. Physiol. 2007;34(5-6):425-431.

    CAS  Article  PubMed  Google Scholar 

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Correspondence to I. I. Bobyntsev.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 4, pp. 407-410, April, 2017

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Fomenko, E.V., Bobyntsev, I.I., Kryukov, A.A. et al. Effect of Selank on Functional State of Rat Hepatocytes under Conditions of Restraint Stress. Bull Exp Biol Med 163, 415–418 (2017). https://doi.org/10.1007/s10517-017-3817-8

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Key Words

  • Selank
  • restraint stress
  • liver
  • free radical oxidation
  • serum aminotransferases