Differential expression of type 1 cannabinoid receptors (CR1) was evaluated at different stages of human skin fibroblast transformation into terminally differentiated neurons. Immunocytochemical staining detected no CR1 on fibroblasts, but their transformation into induced pluripotent stem cells was accompanied by marked stimulation of CR1 expression. In neuronal precursors, the receptors were located mainly on cell bodies and at the base of their processes. This distribution was retained at the terminal stage of differentiation of induced pluripotent stem cells into neurons.
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Lebedeva OS, Novosadova EV, Manuilova ES, Arsen’eva EL, Kiseleca SL, Larar’kova MA, Khaspekov LG, Illarioshkin SN, Grivennikov IA. Preparation and characterization of the cell model of Parkinson’s disease based on induced pluripotent stem cells. Stem Cells and Regenerative Medicine. Moscow, 2014. P. 154-168. Russian.
Berger C, Schmid PC, Schabitz WR, Wolf M, Schwab S, Schmid HH. Massive accumulation of N-acylethanolamines after stroke. Cell signalling in acute cerebral ischemia?. J. Neurochem. 2004;88(5):1159-1167.
Compagnucci C, Di Siena S, Bustamante MB, Di Giacomo D, Di Tommaso M, Maccarrone M, Grimaldi P, Sette C. Type-1 (CB1) cannabinoid receptor promotes neuronal differentiation and maturation of neural stem cells. PLoS One. 2013;8(1):e54271.
Galve-Roperh I, Chiurchiu V, Diaz-Alonso J, Bari M, Guzman M, Maccarrone M. Cannabinoid receptor signaling in progenitor/stem cell proliferation and differentiation. Prog. Lipid Res. 2013;52(4):633-650.
Glass M, Dragunow M, Faull RL. Cannabinoid receptors in the human brain: a detailed anatomical and quantitative autoradiographic study in the fetal, neonatal and adult human brain. Neuroscience. 1997;77(2):299-318.
Hermann H, Marsicano G, Lutz B. Coexpression of the cannabinoid receptor type 1 with dopamine and serotonin receptors in distinct neuronal subpopulations of the adult mouse forebrain. Neuroscience. 2002;109(3):451-460.
Hillard CJ. The endocannabinoid signaling system in the CNS: a primer. Int. Rev. Neurobiol. 2015;125:1-47.
Hwang DY, Kim DS, Kim DW. Human ES and iPS cells as cell sources for the treatment of Parkinson’s disease: current state and problems. J. Cell. Biochem. 2010;109(2):292-301.
Jin K, Xie L, Kim SH, Parmentier-Batteur S, Sun Y, Mao XO, Childs J, Greenberg DA. Defective adult neurogenesis in CB1 cannabinoid receptor knockout mice. Mol. Pharmacol. 2004;66(2):204-208.
Lau T, Schloss P. The cannabinoid CB1 receptor is expressed on serotonergic and dopaminergic neurons. Eur. J. Pharmacol. 2008;578(2-3):137-141.
Mackie K. Distribution of cannabinoid receptors in the central and peripheral nervous system. Handb. Exp. Pharmacol. 2005;(168):299-325.
Ramos JA, Gomez M, de Miguel R. Effects on development. Handb. Exp. Pharmacol. 2005;(168):643-656.
Soltys J, Yushak M, Mao-Draayer Y. Regulation of neural progenitor cell fate by anandamide. Biochem. Biophys. Res. Commun. 2010;400(1):21-26.
Stanslowsky N, Jahn K, Venneri A, Naujock M, Haase A, Martin U, Frieling H, Wegner F. Functional effects of cannabinoids during dopaminergic specification of human neural precursors derived from induced pluripotent stem cells. Addict. Biol. 2016. Mar 30. doi: 10.1111/adb.12394.
Yamanaka S. A fresh look at iPS cells. Cell. 2009;137(1)
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 2, pp. 242-245, February, 2017
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Bobrov, M.Y., Bezuglov, V.V., Khaspekov, L.G. et al. Expression of Type I Cannabinoid Receptors at Different Stages of Neuronal Differentiation of Human Fibroblasts. Bull Exp Biol Med 163, 272–275 (2017). https://doi.org/10.1007/s10517-017-3782-2
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DOI: https://doi.org/10.1007/s10517-017-3782-2