Skip to main content
SpringerLink
Log in

Dynamics of the Pro-Oxidant/Antioxidant System Parameters in Wound Discharge and Plasma in Experimental Purulent Wound during Its Technological Liquid Phase Treatment

  • EXPERIMENTAL METHODS FOR CLINICAL PRACTICE
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The dynamics of the pro-oxidant/antioxidant system of rabbits was studied at the local (in exudate) and systemic (blood) levels during therapy of purulent wounds in liquid medium with the use of a programmed device and the efficiency of the proposed technological method was compared with that of classical methods for the treatment of these wounds. More rapid recovery of the indicators of the pro-oxidant/antioxidant system to physiological values in the postoperative period was observed after treatment by the proposed method (7 days vs. 10 days after standard treatment), these results attest to strengthening of the adaptation potential in laboratory animals. The intensity of free radical oxidation in the exudate decreased by 26.6% in animals treated by the technological method. These data indicated acceleration of the regenerative processes at the local level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aliev MD, Sokolovskii VA, Dmitrieva NV, Sinyukova GT, Sycheva LYu, Amiraskanov AA, Mistakopulo NF. Complications after arthroplasty of large joints. Methods of treatment. Vestn. Ross. Nauch. Tsentr. im. N. N. Blokhina Ross. Akad. Med. Nauk. 2003;14(2-1):35-39. Russian.

    Google Scholar 

  2. Korolyuk MA, Ivanov LI, Maioriva IG, Tokarev VP. A method of measuring catalase activity. Lab. Delo. 1988;(1):16-19.

  3. Kostiuk VA, Potapovich AI, Kovaleva ZhV. A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation. Voprosy Med. Khimii. 1990;(2)88-91.

  4. Makhan’ko AV, Malyshko VV, Basov AA, Fedosov SR, Vlasov RV, Chernobai KN. Certificate of state registration of the computer program No. 2015615207. Device management program for the treatment of wounds. Date of registration May 13, 2015. Russian.

  5. Pavlyuchenko II, Fedosov SR, Basov AA. Certificate of state registration of the computer program No. 2006611562. Program for recording signals from a LT-1 chemiluminotester. Date of registration May 10, 2006. Russian.

  6. Basov AA, Bykov IM, Fedosov SR, Malyshko VV. Patent RF No. 2455703. Method of surgical simulation of oxidative stress in laboratory animals. Bull. No. 19. Published July 10, 2012.

  7. Basov AA, Malyshko VV, Fedosov SR. Patent RF No. 163627. Device for topical treatment and dynamic observation of superficial purulent wounds. Bull. No. 21. Published July 27, 2016. Russian.

  8. Basov AA, Pavljuchenko II, Bykov IM, Fedosov SR, Gubareva EA. Patent RF No. 2436101. Diagnostic technique for metabolic disorders in the body in oxidative stress environment. Bull. No. 34. Published December 10, 2011.

  9. Pavlyuchenko II, Basov AA, Fedosov SR. Patent RF No. 54787. A system for laboratory diagnostics of oxidative stress. Bull. No. 21. Published July 27, 2006. Russian.

  10. Pyatkin OV, Medvedev GM, Pyatkova GV, Vashchenko АV, Pyatkov AA. Optimization of treatment of wound purulent complications in military men of Northern Region. Ekol. Chel. 2006;(6):25-27. Russian.

  11. Fedosov SR, Savchenko YuP, Pavlyuchenko II, Golikov IV, Pyatakov SN. The dynamics of prooxidant-antioxidant balance in the process of septic wound development. Kubansk. Nauch. Med. Vestn. 2010;(9):166-169. Russian.

  12. Surgical Infections of the Skin and Soft Tissues. Russian National Recommendations. Savel’eva VS, ed. Moscow, 2009. Russian.

  13. Badr G, Hozzein WN, Badr BM, Al Ghamdi A, Saad Eldien HM, Garraud O. Bee venom accelerates wound healing in diabetic mice by suppressing activating transcription factor-3 (ATF-3) and inducible nitric oxide synthase (INOS)-mediated oxidative stress and recruiting bone marrow-derived endothelial progenitor cells. J. Cell. Physiol. 2016;231(10):2159-2171.

    Article  CAS  PubMed  Google Scholar 

  14. Van der Vliet A, Janssen-Heininger YM. Hydrogen peroxide as a damage signal in tissue injury and inflammation: murderer, mediator, or messenger? J. Cell. Biochem. 2014;115(3):427-435.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Yashin AY. A flow-injection system with amperometric detection for selective determination of antioxidants in foodstuffs and drinks. Russ. J. General Chem. 2008;78(12):2566-2571.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kuban State Medical University, the Ministry of Health of the Russian Federation, Krasnodar, Russia

    I. M. Bykov, A. A. Basov & V. V. Malyshko

  2. Kuban State University, Krasnodar, Russia

    S. S. Dzhimak & S. R. Fedosov

  3. Kuban State Agrarian University, Krasnodar, Russia

    A. V. Moiseev

Authors
  1. I. M. Bykov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Basov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. V. Malyshko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. S. Dzhimak
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. R. Fedosov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. V. Moiseev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. A. Basov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 2, pp. 237-241, February, 2017

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bykov, I.M., Basov, A.A., Malyshko, V.V. et al. Dynamics of the Pro-Oxidant/Antioxidant System Parameters in Wound Discharge and Plasma in Experimental Purulent Wound during Its Technological Liquid Phase Treatment. Bull Exp Biol Med 163, 268–271 (2017). https://doi.org/10.1007/s10517-017-3781-3

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-017-3781-3

Key Words

Search

Navigation