Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 2, pp. 171-175, February, 2017
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Kostin, N.N., Ponomarenko, N.A., Isaev, V.A. et al. Preparation of Recombinant Serpin from Red King Crab Paralithodes сamtschaticus for Biomedical Research Purposes. Bull Exp Biol Med 163, 210–213 (2017). https://doi.org/10.1007/s10517-017-3768-0
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DOI: https://doi.org/10.1007/s10517-017-3768-0