We analyzed migration of monocytes under the effect of apocinin (NADPH inhibitor) and PD98059 (blocker of extracellular MEK/ERK kinase involved in Nox4 oxidase-mediated migration of monocytes). Migration of monocytes stimulated by cysteine-containing peptides (fragments of chemokines with free thiol group MCP-1 and fractalkine) was completely inhibited by apocinin and MEK/ERK blocker. It is assumed that the stimulating effect of cysteine-containing peptides on monocyte migration is mediated by the NADPH-oxidase system, in particular, Nox4.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 2, pp. 164-166, February, 2017
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Krasnikova, T.L., Arefieva, T.I., Pylaeva, E.A. et al. Cysteine-Containing Peptides Stimulate Monocyte Migration through NADPH-Oxidase Activation. Bull Exp Biol Med 163, 203–205 (2017). https://doi.org/10.1007/s10517-017-3766-2
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DOI: https://doi.org/10.1007/s10517-017-3766-2