Skip to main content
SpringerLink
Log in

Initial Stages of Angiogenesis after Acute Experimental Local Venous Outflow Disturbances and Application of Cell Technologies

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The initial stages of angiogenesis in rats after transcutaneous injection of autologous bone marrow multipotent mesenchymal stromal cells transfected with GFP gene and stained cell membranes in the projection of ligated femoral vein were studied by fluorescent light and confocal laser microscopy. Large clusters of brightly fluorescing elongated fibroblast-like cells were seen in the paravasal tissue and in the postoperative scar and signs of angiogenesis were noted as soon as in 4 days. The injected cells not only formed new vessels, but also integrated into vessels formed by host cells. Some injected cells were phagocytizied by macrophages and the latter started to fluoresce due to the presence of the membrane dye. These macrophages within the specified period appeared in the regional inguinal lymph nodes where they formed clusters in the lymphoid parenchyma of the cortical substance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Borodin YUi, Grigor’ev VN. Lymph Node in Circulatory Disturbances. Novosibirsk, 1986. Russian.

  2. Maiborodin IV, Morozov VV, Novikova YaV, Matveyeva VA, Artemyeva LV, Matveyev AL, Khomeniuk SV, Marchukov SV. Morphological results of stromal stem cells of bone marrow origin into the thrombosed vein in experiment. Morfologiya. 2016;149(2):21-26. Russian.

  3. Maiborodin IV, Morozov VV, Markevich YV, Matveeva VA, Artem’eva LV, Matveev AL, Chastikin GA, Seryapina YV. Acceleration of angiogenesis after paravasal injection of mesenchymal stem cells at the site of modeled venous thrombosis. Bull. Exp. Biol. Med. 2015;159(1):128-133.

    Article  CAS  PubMed  Google Scholar 

  4. Maiborodin IV, Matveyeva VA, Maslov RV, Onopriyenko NV, Kuznetsova IV, Chastikin GA, Anikeyev AA. Some reactions of the regional lymph nodes of rats after implantation of multipotent stromal cells adsorbed on polyhydroxyalkanoate into a bone tissue defect. Morfologiya. 2012;142(4):54-61.

    CAS  Google Scholar 

  5. Campo JJ, Aponte JJ, Nhabomba AJ, Sacarlal J, Angulo-Barturen I, Jimenez-Diaz MB, Alonso PL, Dobano C. Feasibility of flow cytometry for measurements of Plasmodium falciparum parasite burden in studies in areas of malaria endemicity by use of bidimensional assessment of YOYO-1 and autofluorescence. J. Clin. Microbiol. 2011;49(3):968-974.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Li F, Yang M, Wang L, Williamson I, Tian F, Qin M, Shah PK, Sharifi BG. Autofluorescence contributes to false-positive intracellular Foxp3 staining in macrophages: a lesson learned from flow cytometry. J. Immunol. Methods. 2012;386(1-2):101-107.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Mendes-Jorge L, Ramos D, Luppo M, Llombart C, Alexandre-Pires G, Nacher V, Melgarejo V, Correia M, Navarro M, Carretero A, Tafuro S, Rodriguez-Baeza A, Esperança-Pina JA, Bosch F, Ruberte J. Scavenger function of resident autofluorescent perivascular macrophages and their contribution to the maintenance of the blood-retinal barrier. Invest. Ophthalmol. Vis. Sci. 2009;50(12):5997-6005.

    Article  PubMed  Google Scholar 

  8. Mitchell AJ, Pradel LC, Chasson L, Van Rooijen N, Grau GE, Hunt NH, Chimini G. Technical advance: autofluorescence as a tool for myeloid cell analysis. J. Leukoc. Biol. 2010;88(3):597-603.

    Article  CAS  PubMed  Google Scholar 

  9. Potter KA, Simon JS, Velagapudi B, Capadona JR. Reduction of autofluorescence at the microelectrode-cortical tissue interface improves antibody detection. J. Neurosci. Methods. 2012;203(1):96-105.

    Article  CAS  PubMed  Google Scholar 

  10. Watson J. Suppressing autofluorescence of erythrocytes. Biotech. Histochem. 2011;86(3):207. doi: 10.3109/10520295.2011.568971.

    Article  PubMed  Google Scholar 

  11. Wu X, Pan L, Wang Z, Liu X, Zhao D, Zhang X, Rupp RA, Xu J. Ultraviolet irradiation induces autofluorescence enhancement via production of reactive oxygen species and photodecomposition in erythrocytes. Biochem. Biophys. Res. Commun. 2010;396(4):999-1005.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    I. V. Maiborodin, V. V. Morozov, V. A. Matveeva, A. A. Anikeev, R. V. Maslov, G. A. Chastikin & N. F. Figurenko

Authors
  1. I. V. Maiborodin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. V. Morozov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. A. Matveeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Anikeev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. V. Maslov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. A. Chastikin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. N. F. Figurenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. V. Maiborodin.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 27-31, January, 2017

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Maiborodin, I.V., Morozov, V.V., Matveeva, V.A. et al. Initial Stages of Angiogenesis after Acute Experimental Local Venous Outflow Disturbances and Application of Cell Technologies. Bull Exp Biol Med 163, 142–147 (2017). https://doi.org/10.1007/s10517-017-3755-5

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-017-3755-5

Key Words

Search

Navigation