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Internalization of Vectorized Liposomes Loaded with Plasmid DNA in C6 Glioma Cells

  • CELL TECHNOLOGIES IN BIOLOGY AND MEDICINE
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied internalization of vector nanocarriers loaded with plasmid DNA into C6 glioma cells. For improving selectivity of plasmid delivery, the liposomes were conjugated with monoclonal antibodies to VEGF and its receptor VEGFR2. Flow cytofluorometry and laser scanning confocal microscopy showed more intensive (more than 2-fold) internalization and accumulation of antibody-vectorized liposomes in C6 glioma cells in comparison with the control (liposomes conjugated with non-specific antibodies and non-vectorized liposomes). Using quantitative analysis of fluorescent signal, we showed that cationic immunoliposomes significantly more effective delivered pCop-Green-N plasmid DNA and ensured effective transfection of C6 glioma cells.

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Authors and Affiliations

  1. Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Center for Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia

    P. A. Mel’nikov, A. N. Gabashvili, N. V. Nukolova, S. A. Cherepanov, F. A. Koshkin, A. V. Leopol’d & V. P. Chekhonin

  2. Federal Clinical Research Centre, Federal Medical-Biological Agency, Moscow, Russia

    P. A. Mel’nikov & V. P. Baklaushev

  3. Department of Medical Nanobiotechnologies, Medico-Biological Faculty, N. I. Pirogov National Research Medical University, Moscow, Russia

    N. V. Nukolova & V. P. Chekhonin

  4. Department of Chemical Enzymology, Faculty of Biology, M. V. Lomonosov Moscow State University, Moscow, Russia

    I. I. Kuznetsov

Authors
  1. P. A. Mel’nikov
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  2. V. P. Baklaushev
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  3. A. N. Gabashvili
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  4. N. V. Nukolova
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  5. I. I. Kuznetsov
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  6. S. A. Cherepanov
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  7. F. A. Koshkin
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  8. A. V. Leopol’d
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  9. V. P. Chekhonin
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Corresponding author

Correspondence to P. A. Mel’nikov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 3-11, January, 2017

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Mel’nikov, P.A., Baklaushev, V.P., Gabashvili, A.N. et al. Internalization of Vectorized Liposomes Loaded with Plasmid DNA in C6 Glioma Cells. Bull Exp Biol Med 163, 114–122 (2017). https://doi.org/10.1007/s10517-017-3750-x

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  • DOI: https://doi.org/10.1007/s10517-017-3750-x

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