Skip to main content
SpringerLink
Log in

Secretion of Thioredoxin Peroxidase Protein of Cat Liver Fluke Opisthorchis felineus during Modeling of Experimental Opisthorchiasis

  • MICROBIOLOGY AND IMMUNOLOGY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Mechanisms of thioredoxin peroxidase secretion by Opisthorchis felineus were studied in vivo and in vitro. Specific antibodies were obtained and used for western blotting and immunohistochemical detection in Syrian hamster model of opisthorchiasis. Secreted thioredoxin peroxidase protein was accumulated in the worm incubation medium under conditions of oxidative stress and in bile duct cells of hamsters with chronic opisthorchiasis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lvova MN, Duzhak TG, Tsentalovich YuP, Katokhin AV, Mordvinov VA. Secretome of the adult liver fluke Opisthorchis felineus. Parazitologiya. 2014;48(3):169-184. Russian.

  2. Dalton JP, Robinson MW, Mulcahy G, O’Neill SM, Donnelly S. Immunomodulatory molecules of Fasciola hepatica: Candidates for both vaccine and immunotherapeutic development. Vet. Parasitol. 2013;195(3-4):272-285.

    Article  CAS  PubMed  Google Scholar 

  3. Kovner AV, Potapova OV, Shkurupy VA, Shestopalov AM. Morphofunctional Characteristics of pulmonary surfactant system and its effect on immune cells in influenza A (H1N1) pathogenesis. Open J. Pathol. 2016;6(1):1-7. doi: 10.4236/ojpathology.2016.61001.

    Article  Google Scholar 

  4. Maksimova GA, Pakharukova MY, Kashina EV, Zhukova NA, Kovner AV, Lvova MN, Katokhin AV, Tolstikova TG, Sripa B, Mordvinov VA. Effect of opisthorchis felineus infection and dimethylnitrosamine administration on the induction of cholangiocarcinoma in Syrian hamsters. Parasitol. Int. 2015. Oct 6. pii: S1383-5769(15)00166-X. doi: 10.1016/j.parint.2015.10.002.

  5. Maksimova GA, Pakharukova MY, Kashina EV, Zhukova NA, Lvova MN, Khvostov MV, Baev DS, Katokhin AV, Tolstikova TG, Mordvinov VA. The morphofunctional and biochemical characteristics of opisthorchiasis-associated cholangiocarcinoma in a Syrian hamster model. Russ. J. Genet. Appl. Res. 2016;6(4):454-462. doi: 10.18699/VJ15.062.

    Article  CAS  Google Scholar 

  6. McGonigle S, Curley GP, Dalton JP. Cloning of peroxidoxin, a novel antioxidant enzyme from the helminth parasite Fasciola hepatica. Parasitology. 1997;115(Pt 1):101-104.

    Article  CAS  PubMed  Google Scholar 

  7. Ogorodova LM, Fedorova OS, Sripa B, Mordvinov VA, Katokhin AV, Keiser J, Odermatt P, Brindley PJ, Mayboroda OA, Velavan TP, Freidin MB, Sazonov AE, Saltykova IV, Pakharukova MY, Kovshirina YV, Kaloulis K, Krylova OY, Yazdanbakhsh M; TOPIC Consortium. Opisthorchiasis: an overlooked danger. PLoS Negl. Trop. Dis. 2015;9(4):e0003563.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Pakharukova MY, Mordvinov VA. The liver fluke Opisthorchis felineus: biology, epidemiology, and carcinogenic potential. Trans. R Soc. Trop. Med. Hyg. 2016;110(1):28-36.

    Article  PubMed  Google Scholar 

  9. Pakharukova MY, Shilov AG, Pirozhkova DS, Katokhin AV, Mordvinov VA. The first comprehensive study of praziquantel effects in vivo and in vitro on European liver fluke Opisthorchis felineus (Trematoda). Int. J. Antimicrob. Agents. 2015;46(1):94-100.

    Article  CAS  PubMed  Google Scholar 

  10. Sripa B, Kaewkes S, Sithithaworn P, Mairiang E, Laha T, Smout M, Pairojkul C, Bhudhisawasdi V, Tesana S, Thinkamrop B, Bethony JM, Loukas A, Brindley PJ. Liver fluke induces cholangiocarcinoma. PLoS Med. 2007;4(7):e201.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Sripa B, Brindley PJ, Mulvenna J, Laha T, Smout MJ, Mairiang E, Bethony JM, Loukas A. The tumorigenic liver fluke Opisthorchis viverrini ― multiple pathways to cancer. Trends Parasitol. 2012;28(10):395-407.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Suttiprapa S, Loukas A, Laha T, Wongkham S, Kaewkes S, Gaze S, Brindley PJ, Sripa B. Characterization of the antioxidant enzyme, thioredoxin peroxidase, from the carcinogenic human liver fluke, Opisthorchis viverrini. Mol. Biochem. Parasitol. 2008;160(2):116-122.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Federal Research Centre Institute of Cytology and Genetics of the Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    V. A. Petrenko, M. Yu. Pakharukova, A. V. Kovner, M. N. L’vova & V. A. Mordvinov

  2. Research Institute of Molecular Biology and Biophysics, Novosibirsk, Russia

    M. Yu. Pakharukova, V. V. Lyakhovich & V. A. Mordvinov

Authors
  1. V. A. Petrenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Yu. Pakharukova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. V. Kovner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. N. L’vova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. V. Lyakhovich
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. A. Mordvinov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Yu. Pakharukova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 12, pp. 743-747, December, 2016

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Petrenko, V.A., Pakharukova, M.Y., Kovner, A.V. et al. Secretion of Thioredoxin Peroxidase Protein of Cat Liver Fluke Opisthorchis felineus during Modeling of Experimental Opisthorchiasis. Bull Exp Biol Med 162, 773–776 (2017). https://doi.org/10.1007/s10517-017-3710-5

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-017-3710-5

Key Words

Search

Navigation