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Influence of Humic Acids Extracted from Peat by Different Methods on Functional Activity of Macrophages in Vitro

  • PHARMACOLOGY AND TOXICOLOGY
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We studied activation of macrophages with humic acids extracted from peat of large deposits in the Tomsk region by two extraction methods: by hydroxide or sodium pyrophosphate. Humic acid of lowland peat types containing large amounts of aromatic carbon, phenolic and alcohol groups, carbohydrate residues and ethers, irrespectively of the extraction methods contained LPS admixture that probably determines their activating properties. Humic acid of upland peat types characterized by high content of carbonyl, carboxyl, and ester groups enhance NO production and reduce arginase expression, but these effects were minimized when sodium hydroxide was used as an extraction solvent. Pyrophosphate samples of the upland peat types were characterized by aromaticity and diversity of functional groups and have a significant advantage because of they induce specific endotoxin-independent stimulating action on antigen presenting cells.

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Authors and Affiliations

  1. E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Centre, Tomsk, Russia

    E. S. Trofimova, A. A. Ligacheva, E. Y. Sherstoboev, V. V. Zhdanov, M. G. Danilets & A. M. Dygai

  2. Siberian State Medical University, Tomsk, Russia

    M. V. Zykova, M. V. Belousov, M. S. Yusubov & S. V. Krivoshchekov

  3. National Research Tomsk Polytechnic University, Tomsk, Russia

    M. S. Yusubov & S. V. Krivoshchekov

Authors
  1. E. S. Trofimova
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  2. M. V. Zykova
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  3. A. A. Ligacheva
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  4. E. Y. Sherstoboev
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  5. V. V. Zhdanov
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  6. M. V. Belousov
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  7. M. S. Yusubov
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  8. S. V. Krivoshchekov
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  9. M. G. Danilets
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  10. A. M. Dygai
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Corresponding author

Correspondence to M. G. Danilets.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 12, pp. 708-713, December, 2016

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Trofimova, E.S., Zykova, M.V., Ligacheva, A.A. et al. Influence of Humic Acids Extracted from Peat by Different Methods on Functional Activity of Macrophages in Vitro . Bull Exp Biol Med 162, 741–745 (2017). https://doi.org/10.1007/s10517-017-3702-5

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  • DOI: https://doi.org/10.1007/s10517-017-3702-5

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