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Antithrombin Activity of Erythrocyte Microvesicles

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Coagulation and optical (based on chromogenic substrate) methods were employed to examine antithrombin activity of erythrocytes and erythrocyte-derived microvesicles isolated days 7, 14, 21, and 28 on erythrocyte storage. The erythrocyte-derived microvesicles decelerated fibrin clot formation from fibrinogen in the presence of exogenous thrombin both with and without heparin. Microvesicles reduced optical density of chromogenic substrate. These data suggest that erythrocyte-derived microvesicles display a prominent antithrombin activity, which significantly increases during erythrocyte storage.

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Authors and Affiliations

  1. Department of Gravitational Surgery and Hemodialysis, Volga Federal Medical Research Center, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia

    G. Ya. Levin & E. G. Sukhareva

Authors
  1. G. Ya. Levin
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  2. E. G. Sukhareva
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Correspondence to G. Ya. Levin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 12, pp. 682-685, December, 2016

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Levin, G.Y., Sukhareva, E.G. Antithrombin Activity of Erythrocyte Microvesicles. Bull Exp Biol Med 162, 718–721 (2017). https://doi.org/10.1007/s10517-017-3696-z

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  • DOI: https://doi.org/10.1007/s10517-017-3696-z

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