• Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting

Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.

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Correspondence to A. M. Subbot.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 248-253, October, 2016

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Novikov, I.A., Vakhrushev, I.V., Antonov, E.N. et al. Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting. Bull Exp Biol Med 162, 558–562 (2017). https://doi.org/10.1007/s10517-017-3659-4

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Key Words

  • scanning electron microscopy
  • lanthanide
  • mesenchymal stromal cells
  • tissue engineering