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Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 4, pp 462–465 | Cite as

Effect of Sulfation and Molecular Weight on Anticoagulant Activity of Dextran

  • N. N. Drozd
  • Yu. S. Logvinova
  • M. A. Torlopov
  • E. V. Udoratina
Article
  • 60 Downloads

Sulfation (to 2.8) of dextrans with molecular weight of 150 and 20 kDa was followed by the appearance of anticoagulant activity that increased with decreasing their molecular weight and did not depend on antithrombin, plasma inhibitor of serine proteases of the blood coagulation system. Antithrombin activity of dextran sulfate with a molecular weight of 20 kDa reached 12.6-15.3 U/mg. Dextran sulfates with molecular weights of 20 and 150 kDa did not potentiate ADP-induced human platelet aggregation.

Key Words

dextran dextran sulfate anticoagulant activity 

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References

  1. 1.
    Makarov VA, Spasov AA, Plotnikova MB, Belozerskaya GG, Vasil’eva TM, Drozd NN, svistunov AA, Kucheryavenko AF, Malykhina LS, Naumenko LV, Nevedrova OE, Petrukhina GN, Aliev OI, Plotnikova TM. Methodical recommendations for testing drugs affecting hemostasis. Manual for Preclinical Studies of New Pharmacological Substances. Part I, Mironov AN, ed. Moscow, 2012. P. 453-479. Russian.Google Scholar
  2. 2.
    Born GV. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962;194:927-929.CrossRefPubMedGoogle Scholar
  3. 3.
    de Raucourt E, Mauray S, Chaubet F, Maiga-Revel O, Jozefowicz M, Fischer A.M. Anticoagulant activity of dextran derivatives. J. Biomed. Mater. Res. 1998;41(1):49-57.CrossRefPubMedGoogle Scholar
  4. 4.
    Jones CI, Payne DA, Hayes PD, Naylor AR, Bell PR, Thompson MM, Goodall AH. The antithrombotic effect of dextran-40 in man is due to enhanced fibrinolysis in vivo. J. Vasc. Surg. 2008;48(3):715-722.CrossRefPubMedGoogle Scholar
  5. 5.
    Robless P, Okonko D, Mikhailidis DP, Stansby G. Dextran 40 reduces in vitro platelet aggregation in peripheral arterial disease. Platelets. 2004;15(4):215-222.CrossRefPubMedGoogle Scholar
  6. 6.
    Saito A. Heparin cofactor II is degraded by heparan sulfate and dextran sulfate. Biochem. Biophys. Res. Commun. 2015;457(4):585-588.CrossRefPubMedGoogle Scholar
  7. 7.
    Teien AN, Lie M. Evaluation of an amidolytic heparin assay method: increased sensitivity by adding purified antithrombin III. Thromb. Res. 1977;10(3):399-410.CrossRefPubMedGoogle Scholar
  8. 8.
    Yu P, Gu H. Bioactive substances from marine fishes, shrimps, and algae and their functions: present and future. Crit. Rev. Food. Sci. Nutr. 2015;55(8):1114-1136.CrossRefPubMedGoogle Scholar
  9. 9.
    Zeerleder S, Mauron T, Lämmle B, Wuillemin WA. Effect of low-molecular weight dextran sulfate on coagulation and platelet function tests. Thromb. Res. 2002;105(5):441-446.CrossRefPubMedGoogle Scholar
  10. 10.
    Zhu F, Heditke S, Kurtzberg J, Waters-Pick B, Hari P, Margolis DA, Keever-Taylor CA. Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products. Cytotherapy. 2015;17(12):1813-1819.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • N. N. Drozd
    • 1
  • Yu. S. Logvinova
    • 1
  • M. A. Torlopov
    • 2
  • E. V. Udoratina
    • 2
  1. 1.National Research Center for Hematology, Ministry of Health of the Russian FederationMoscowRussia
  2. 2.Institute of Chemistry, Komi Science CenterUral Division of the Russian Academy of SciencesSyktyvkarRussia

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