Skip to main content
SpringerLink
Log in

Changes in the Neurochemical Composition of Motor Neurons of the Spinal Cord in Mice under Conditions of Space Flight

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Expression of choline acetyltransferase, 200-kDa neurofilament protein, 28-kDa calbindin, neuronal NO synthase, caspase 3, and Ki-67 in the motor neurons of spinal cord segments T3-T5 in male C57Bl/6 mice after 30-day space flight in the Bion-M1 biosatellite was studied by immunohistochemical methods. Under conditions space flight, the size of motoneurons increased, the number of neurons containing choline acetyltransferase and neurofilaments, decreased, and the number of calbindin-positive neurons increased; motoneurons, expressing neuronal NO synthase and caspase 3 appeared, while Ki-67 was not detected. Fragmentation of neurons with the formation structures similar to apoptotic (residual) bodies was observed in individual caspase 3-positive motoneurons.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Andreev-Andrievsky AA, Shenkman BS, Popova AS, Dolguikh ON, Anokhin KV, Soldatov PE, Ilyin EA, Sychev VN. Experimental studies with mice on the program of the biosatellite BION-M1 mission. Aviakosm. Ekol. Med. 2014;48(1):14-27. Russian.

    CAS  Google Scholar 

  2. Islamov RR, Valiullin VV, Nikolsky EE, Tyapkina OV, Bukharaeva EA, Yagodina LO, Ibragimova NN, Kozlovskaya IB. Expression of choline acetyltransferase in rat spinal motoneurons after antiorthostatic suspemsion. Doklady Biol. Sci. 2007;414(1):205-207.

    Article  CAS  Google Scholar 

  3. Krasnov IB. Deafferentation and commutation change of brain structures in weightlessness. Aviakosm. Ekol. Med. 2013;47(4):83-84. Russian.

    Google Scholar 

  4. Polyakov IV, Louri O, Edzherton VP, Krasnov IB. Histochemistry and morphology of the anterior horns of the spinal cord in rats after 9-day spaceflight Aviakosm. Ekol. Med. 1995;29(1):30-32. Russian.

    Google Scholar 

  5. Porseva VV, Shilkin VV, Strelkov AA, Masliukov PM. Calbindin-containing neurons of the ventral horn of murine spinal cord gray matter. Morfologiya. 2014;146(4):21-25. Russian.

    CAS  Google Scholar 

  6. Porseva VV, Shilkin VV, Strelkov AA, Masliukov PM. Subpopulation of calbindin-immunoreactive innrerneurons in toe dorsal horn of toe mice spinal cord. Tsitologiya. 2014;56(8):612-618. Russian.

    CAS  Google Scholar 

  7. Fuchs A, Lirk P, Stucky C, Abram SE, Hogan QH. Painful nerve injury decreases resting cytosolic calcium concentrations in sensory neurons of rats. Anesthesiology. 2005;102(6):1217-1225.

    Article  CAS  PubMed  Google Scholar 

  8. Gibbons SJ, Brorson JR, Bleakman D, Chard PS, Miller RJ. Calcium influx and neurodegeneration. Ann. N.Y. Acad. Sci. 1993;679:22-33.

    Article  CAS  PubMed  Google Scholar 

  9. Ishihara A, Ohira Y, Roy RR, Nagaoka S, Sekiguchi C, Hinds WE, Edgerton VR. Comparison of the response of motoneurons innervating perineal and hind limb muscles to spaceflight and recovery. Muscle Nerv. 2000;23(5):753-762.

    Article  CAS  Google Scholar 

  10. Kim KH, Kim JI, Han JA, Choe MA, Ahn JH. Upregulation of neuronal nitric oxide synthase in the periphery promotes pain hypersensitivity after peripheral nerve injury. Neuroscience. 2011;190:367-378.

    Article  CAS  PubMed  Google Scholar 

  11. Kozlovskaya IB, Sayenko IV, Sayenko DG, Miller TF, Khusnutdinova DR, Melnik KA. Role of support afferentation in control of the tonic muscle activity. Acta Astronaut. 2007;60(4-7):285-294.

    Article  Google Scholar 

  12. Krasnov IB. Gravitational neuromorphology. Adv. Space Biol. Med. 1994;4:85-110.

    Article  CAS  PubMed  Google Scholar 

  13. Masliukov PM, Emanuilov AI, Madalieva LV, Moiseev KY, Bulibin AV, Korzina MB, Porseva VV, Korobkin AA, Smirnova VP. Development of nNOS-positive neurons in the rat sensory and sympathetic ganglia. Neuroscience. 2014;256:271-281.

    Article  CAS  PubMed  Google Scholar 

  14. Perečko T, Drábiková K, Nosáľ R, Harmatha J, Jančinová V. Involvement of caspase 3 in stilbene derivatives induced apoptosis of human neutrophils in vitro. Interdiscip. Toxicol. 2012;5(2):76-80.

    PubMed  PubMed Central  Google Scholar 

  15. Schwaller B. The use of transgenic mouse models to reveal the functions of Ca2+ buffer proteins in excitable cells. Biochim. Biophys. Acta. 2012;1820(8):1294-1303.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia

    V. V. Porseva, V. V. Shilkin, A. A. Strelkov & P. M. Masliukov

  2. Laboratory of Gravitational Biology, State Scientific Centre Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    I. B. Krasnov

Authors
  1. V. V. Porseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. V. Shilkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. A. Strelkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. B. Krasnov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. M. Masliukov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. M. Masliukov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 9, pp. 318-321, September, 2016

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Porseva, V.V., Shilkin, V.V., Strelkov, A.A. et al. Changes in the Neurochemical Composition of Motor Neurons of the Spinal Cord in Mice under Conditions of Space Flight. Bull Exp Biol Med 162, 336–339 (2017). https://doi.org/10.1007/s10517-017-3609-1

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-017-3609-1

Key Words

Search

Navigation