Subcutaneous injection of Oxacom with glutathione-bound dinitrosyl iron complex as the active principle produced a slower drop of mean BP and longer accumulation of protein-bound dinitrosyl iron complexes in whole blood and tissues than intravenous injection of this drug, while durations of hypotensive effect in both cases were practically identical. In contrast to intravenous injection of the drug, its subcutaneous administration was not characterized by a high concentration of protein-bound dinitrosyl iron complexes in the blood at the onset of experiment; in addition, accumulation of these NO forms in the lungs was more pronounced after subcutaneous injection than after intravenous one.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Vanin AF. Dinitrosyl Iron Complex with Thiol-Containing Ligands. Physical Chemistry, Biology, Medicine. Moscow; Izhevsk, 2015. Russian.
Gosteev AIu, Zorin AV, Rodnenkov OV, Dragnev AG, Chazov EI. Hemodynamic effects of the synthetic analogue of endogenous nitric oxide (II) donors a dinitrosyl iron complex in hypertensive patients with uncomplicated hypertensive crisis. Ter. Arkhiv. 2014;86(9):49-55. Russian.
Timoshin AA, Drobotova DY, Tskitishvili OV, Serebryakova LI, Pisarenko OI, Ruuge EK, Vanin AF. Protective effect of dinitrosyl-iron complexes with glutathione in rat myocardial regional ischemia: A microdialysis assay study. Dokl. Biochem. Biophysics. 2010;432(3):106-109.
Timoshin AA, Lakomkin VL, Abramov AA, Ruuge EK, Vanin AF. Effect of dinitrosyl iron complexes on NO level in rat organs during endotoxin shock. Dokl. Biochem. Biophysics. 2015;462(2):166-168.
Timoshin AA, Lakomkin VL, Ruuge EK, Vanin AF. Distribution and pharmacokinetics of dinitrosyl iron complexes in rat organs. Biophysics. 2012;57(2):233-236.
Chazov EI, Rodnenkov OV, Zorin AV, Lakomkin VL, Gramovich VV, Vyborov ON, Dragnev AG, Timoshin AA, Buryachkovskaya LI, Abramov AA, Massenko VP, Arzamastsev EV, Kapelko VI, Vanin AF. Hypotensive effect of Oxacom_containing a dinitrosyl iron complex with glutathione: animal studies and clinical trials on healthy volunteers. Nitric Oxide. 2012;26(3):148-156.
Flitney FW, Megson IL, Flitney DE, Butler AR. Iron-sulfur clustrer nitrosyls, a novel class of nitric oxide generator: mechanism of vasodilator action on rat isolated tail artery. Br. J. Pharmacol. 1992;107(3):842-848.
Lakomkin VL, Vanin AF, Timoshin AA, Kapelko VI, Chazov EI. Long-lasting hypotensive action of stable preparations of dinitrosyl-iron complexes with thiol-containing ligands in conscious normotensive and hypertensive rats. Nitric Oxide. 2007;16(4):413-418.
Lancaster JR Jr, Werner-Felmayer G, Wachter H. Coinduction of nitric oxide synthesis and intracellular nonheme ironnitrosyl complexes in murine cytokine-treated fibroblasts. Free Radic. Biol. Med. 1994;16(6):869-870.
Mülsch A, Mordvintcev PI, Vanin AF, Busse R. Formation and release of dinitrosyl iron complexes by endothelial cells. Biochem. Biophys. Res. Commun. 1993;196(3):1303-1308.
Shumaev KB, Gubkin AA, Serezhenkov VA, Lobysheva II, Kosmachevskaya OV, Ruuge EK, Lankin VZ, Topunov AF, Vanin AF. Interaction of reactive oxygen and nitrogen species with albumin- and methemoglobin-bound dinitrosyl-iron complexes. Nitric Oxide. 2008;18(1):37-46.
Timoshin AA, Lakomkin VL, Abramov AA, Ruuge EK, Kapel’ko VI, Chazov EI, Vanin AF. The hypotensive effect of the nitric monoxide donor Oxacom at different routs of its administration to experimental animals. Eur. J. Pharmacol. 2015;765:525-532.
Vanin AF. Dinitrosyl iron complexes with thiolate ligands: physico-chemistry, biochemistry and physiology. Nitric Oxide. 2009;21(1):1-13.
Vanin AF, Poltorakov AP, Mikoyan VD, Kubrina LN, Burbaev DS. Polynuclear water-soluble dinitrosyl iron complexes with cysteine and glutathione ligands: electron paramagnetic resonance and optical studies. Nitric Oxide. 2010;23(2):136-149.
Watts RN, Hawkins C, Ponka P, Richardson DR. Nitrogen monoxide (NO)-mediated iron release from cells is linked with NOinduced glutathione efflux via multidrug resistance-associated protein. Proc. Natl. Acad. Sci. USA. 2006;103(20):7670-7675.
Author information
Authors and Affiliations
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 8, pp. 169-172, August 2016
Rights and permissions
About this article
Cite this article
Timoshin, A.A., Lakomkin, V.L., Abramov, A.A. et al. Hypotensive Effect and Accumulation of Dinitrosyl Iron Complexes in Blood and Tissues after Intravenous and Subcutaneous Injection. Bull Exp Biol Med 162, 207–210 (2016). https://doi.org/10.1007/s10517-016-3577-x
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3577-x