We analyzed the effects of hypoxia and reoxygenation on changes in contractile activity in rat aortic smooth muscles. Both hypoxia and reoxygenation induced relaxation of smooth muscle cells precontracted with high-potassium Krebs solution (30 mM KCl) or α1-adrenoceptor agonist phenylephrine. Vasodilation resulted from enhancement of potassium permeability of smooth muscle cell membranes caused by activation of voltage-gated potassium channels (triggered by both precontracting agents) or by opening of ATP-sensitive potassium channels (phenylephrine). In isolated smooth muscle cells, both hypoxia and inhibition of Na+,K+-ATPase with ouabain led to depletion of intracellular store of macroergic substances, reduced potassium concentration, and elevated the content of sodium ions.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 8, pp. 157-160, August 2016
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Gusakova, S.V., Birulina, Y.G., Smagliy, L.V. et al. Regulation of Contractile Responses of Vascular Smooth Muscle Cells under Conditions of Hypoxia—Reoxygenation. Bull Exp Biol Med 162, 195–198 (2016). https://doi.org/10.1007/s10517-016-3574-0
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DOI: https://doi.org/10.1007/s10517-016-3574-0