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The Use of Technetium-99m for Intravital Tracing of Transplanted Multipotent Stromal Cells

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the possibility of in vivo tracing of multipotent mesenchymal stromal cells labeled with a radiophermaceutic preparation based on metastable isotope Technetium-99m and injected to rats with modeled traumatic brain injury. Accumulation of labeled cells occurred primarily in the liver and lungs. The cells distribution in internal organs greatly varied depending on the administration route. Cell injection into the carotid artery led to their significant accumulation in the damaged brain hemisphere, while intravenous injection was followed by diffuse cell distribution in all brain structures. Scintigraphy data were confirmed by magnetic resonance imaging and histological staining of cells. Visualization of stem cells labeled with Technetium-99m-based preparation by scintigraphy is an objective and highly informative method allowing real-time in vivo cell tracing in the body.

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Authors and Affiliations

  1. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    D. N. Silachev, E. Yu. Plotnikov, D. B. Zorov & G. T. Sukhikh

  2. A. N. Belozersky Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, Moscow, Russia

    D. N. Silachev, T. I. Danilina, V. N. Manskikh, E. Yu. Plotnikov & D. B. Zorov

  3. N. I. Pirogov National Russian Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia

    A. K. Kondakov & I. A. Znamenskii

  4. Hospital for Incurable Patients — Research Medical Rehabilitation Center, Moscow, Russia

    A. K. Kondakov & I. A. Znamenskii

  5. National Research Nuclear University MEPhI, Moscow, Russia

    Yu. B. Kurashvili

  6. P. A. Hertsen Moscow Oncology Research Institute, Moscow, Russia

    Yu. B. Kurashvili, A. V. Abolenskaya & N. R. Antipkin

  7. Laboratory of Magnetic Tomography and Spectroscopy, Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia

    M. V. Gulyaev & Yu. A. Pirogov

Authors
  1. D. N. Silachev
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  2. A. K. Kondakov
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  3. I. A. Znamenskii
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  4. Yu. B. Kurashvili
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  5. A. V. Abolenskaya
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  6. N. R. Antipkin
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  7. T. I. Danilina
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  8. V. N. Manskikh
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  9. M. V. Gulyaev
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  10. Yu. A. Pirogov
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  11. E. Yu. Plotnikov
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  12. D. B. Zorov
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  13. G. T. Sukhikh
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Corresponding author

Correspondence to D. B. Zorov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 188-195, July, 2016

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Silachev, D.N., Kondakov, A.K., Znamenskii, I.A. et al. The Use of Technetium-99m for Intravital Tracing of Transplanted Multipotent Stromal Cells. Bull Exp Biol Med 162, 153–159 (2016). https://doi.org/10.1007/s10517-016-3565-1

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  • DOI: https://doi.org/10.1007/s10517-016-3565-1

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