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Analysis of Reparative Activity of Platelet Lysate: Effect on Cell Monolayer Recovery In Vitro and Skin Wound Healing In Vivo

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Platelet lysate prepared from donor platelet concentrate and pooled according to a developed technique stimulates migration of multipotent mesenchymal stromal cells of the human adipose tissue and promotes healing of the monolayer defect in cultures of human fibroblasts and multipotent mesenchymal stromal cells in vitro in concentrations close those of fetal calf serum (5-10%). Lysate of platelets from platelet-rich rat blood plasma stimulated healing of the skin defect by promoting epithelialization and granulation tissue formation. The regenerative properties of platelet lysate in vivo increased with increasing its concentration.

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Authors and Affiliations

  1. P. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Ministry of Health of the Russian Federation, Moscow, Russia

    N. S. Sergeeva, Ya. D. Shanskii, I. K. Sviridova, P. A. Karalkin, V. A. Kirsanova, S. A. Akhmedova & A. D. Kaprin

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  1. N. S. Sergeeva
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  2. Ya. D. Shanskii
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  3. I. K. Sviridova
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  4. P. A. Karalkin
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  5. V. A. Kirsanova
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  6. S. A. Akhmedova
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  7. A. D. Kaprin
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Corresponding author

Correspondence to N. S. Sergeeva.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 172-179, July, 2016

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Sergeeva, N.S., Shanskii, Y.D., Sviridova, I.K. et al. Analysis of Reparative Activity of Platelet Lysate: Effect on Cell Monolayer Recovery In Vitro and Skin Wound Healing In Vivo . Bull Exp Biol Med 162, 138–145 (2016). https://doi.org/10.1007/s10517-016-3563-3

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  • DOI: https://doi.org/10.1007/s10517-016-3563-3

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