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Inhibition of Tobacco Ringspot Virus by the Culture Fluid of L-Lysine-α-Oxidase Producing Strain

  • VIROLOGY
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Bulletin of Experimental Biology and Medicine Aims and scope

A producing strain of an anti-tumor and antiviral enzyme L-lysine-α-oxidase from Trichoderma was cultured using a technological device of G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (Pushchino). L-lysine-α-oxidase activity in the obtained metabolite concentrate was 5.4 U/ml. We studied the effects of the concentrate of active L-lysine-α-oxidase producer on the highly infectious Tobacco ringspot virus and revealed anti-viral activity of it when enzyme concentration was at least 1.0 U/ml.

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References

  1. Selytsheva AA, Alexeev SB, Smirnova IP, Podboronov VM. Antiherpetic activity of L-lysine-α-oxydase in different dosage forms. Antibiotiki Khimioterapiya. 2003;48(1):9-12. Russian.

    CAS  Google Scholar 

  2. Smirnova IP, Alekseev SB, Podboronov VM. Study of the effect of L-lysine-α-oxidase gel on development of eye and skin herpetic lesions in rabbits. Antibiotiki Khimioterapiya. 2003;48(11):7-9. Russian.

    CAS  Google Scholar 

  3. Smirnova IP, Alekseev SB, Shevchenko AA. Biosynthesis of L-lysine-a-oxidase, an antitumor enzyme, by Trichoderma spp. Antibiotiki Khimioterapiya. 2009;54(5-6):8-12. Russian.

    CAS  Google Scholar 

  4. Smirnova IP, Berezov TT. Biotechnology of L-Lysine-α-Oxidase from Trichoderma. Moscow, 2014. Russian.

  5. Smirnova IP, Syatkin SP, Berezov TT. Spectrophotometrical L-lysine-α-oxidase microassay. Vopr. Med. Khimii. 1984; 30(1):133-136. Russian.

    CAS  PubMed  Google Scholar 

  6. Patent RF No. 2475528. Strain Trichoderma harzianum Rifai – Producer of Inhibitor of Tobacco ringspot Virus. Smirnova IP, Shnejder JA. Bull. No. 5. Published February 20, 2013.

  7. Fuchs M, Abawi GS, Marsella-Herrick P, Cox R, Cox KD, Carroll J.E, Martin R.R. Occurrence of tomato ringspot virus and Tobacco ringspot virus in highbush blueberry in New York state. J. Plant Pathol. 2010;92(2):451-459. doi: 10.4454/jpp.v92i2.189.

    CAS  Google Scholar 

  8. OEPP/EPPO (1997) EPPO Standard PM 4/20 (1) Certification scheme for pathogen-ested material of New Guinea hybrids of impatiens. Bulletin OEPP/EPPO Bulletin 27:611-620.

    Article  Google Scholar 

  9. Smirnova I.P, Kuznetsova O.M, Ivanova-Radkevich V.I, Orlova V.S, Podboronov VM, Alexeev AA. Testing of an antitumor enzyme L-lysine-A-oxidase from Trichoderma harzianum Rifai F-180. World J. Med. Sci. 2014;11(2):233-236. doi: 10.5829/idosi.wjms.2014.11.2.84116.

    Google Scholar 

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Authors and Affiliations

  1. Medical Faculty, Medical Institute, Peoples’ Friendship University of Russia, Moscow, Russia

    Yu. A. Shneider, I. P. Smirnova & E. V. Karimova

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  1. Yu. A. Shneider
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  2. I. P. Smirnova
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  3. E. V. Karimova
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Correspondence to I. P. Smirnova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 7, pp. 92-94, July, 2016

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Shneider, Y.A., Smirnova, I.P. & Karimova, E.V. Inhibition of Tobacco Ringspot Virus by the Culture Fluid of L-Lysine-α-Oxidase Producing Strain. Bull Exp Biol Med 162, 79–81 (2016). https://doi.org/10.1007/s10517-016-3550-8

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  • DOI: https://doi.org/10.1007/s10517-016-3550-8

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