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Effect of Cardiac Myosin-Binding Protein C on Tropomyosin Regulation of Actin—Myosin Interaction Using In Vitro Motility Assay

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied the modulating role of cardiac myosin-binding protein C (cMyBP-C) in tropomyosin regulation of the actin—myosin interaction. The effect of cMyBP-C on the velocity of actin-tropomyosin filament sliding over cardiac and slow skeletal myosins was evaluated using in vitro motility assay. The effect of cMyBP-C on the actin-tropomyosin filaments sliding depended on the type of myosin. The regulatory effect of cMyBP-C differs for cardiac and slow skeletal myosin because of the presence of specific essential light chain (LC1sa) in slow skeletal myosin isoform.

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Authors and Affiliations

  1. Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, Ekaterinburg, Russia

    D. V. Shchepkin, G. V. Kopylova & L. V. Nikitina

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  1. D. V. Shchepkin
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  2. G. V. Kopylova
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  3. L. V. Nikitina
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Correspondence to L. V. Nikitina.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 7, pp. 54-57, July, 2016

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Shchepkin, D.V., Kopylova, G.V. & Nikitina, L.V. Effect of Cardiac Myosin-Binding Protein C on Tropomyosin Regulation of Actin—Myosin Interaction Using In Vitro Motility Assay. Bull Exp Biol Med 162, 45–47 (2016). https://doi.org/10.1007/s10517-016-3541-9

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  • DOI: https://doi.org/10.1007/s10517-016-3541-9

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