We studied biological effects of mannan, a polysaccharide immunomodulator from C. albicans, that interacts with mannose receptor in vivo. It is shown that preliminary administration of mannan (5 times in a dose of 50 mg/kg or 2 times in a dose of 100 mg/kg) to mice with acute lipemia induced by lipase inhibitor poloxamer 407 (300 mg/kg) reduces the serum concentrations of atherogenic LDL, cholesterol, and triglycerides. Administration of mannan to intact mice and animals with acute lipemia reduces triglyceride concentration and causes labilization of lysosomal membranes in the liver. Serum activity of chitotriosidase, a marker of macrophage activation, was elevated in mice with acute lipemia treated with mannan. Thus, mannan from C. albicans is a promising hypolipidemic polysaccharide compound, similar by its activity to β-glycan, a component of LPS.
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References
Fraser DA, Tenner AJ. Innate immune proteins C1q and mannan-binding lectin enhance clearance of atherogenic lipoproteins by human monocytes and macrophages. J. Immunol. 2010;185(7):3932-3939.
González R, Ramón D, Pérez-González JA. Cloning, sequence analysis and yeast expression of the egl1 gene from Trichoderma longibrachiatum. Appl. Microbiol. Biotechnol. 1992;38(3):370-375.
Hossain E, Ota A, Karnan S, Takahashi M, Mannan S.B, Konishi H, Hosokawa Y. Lipopolysaccharide augments the uptake of oxidized LDL by up-regulating lectin-like oxidized LDL receptor-1 in macrophages. Mol. Cell Biochem. 2015;400(1-2):29-40.
Hronská L, Mrózová Z, Valachovic M, Hapala I. Low concentrations of the non-ionic detergent Nonidet P-40 interferewith sterol biogenesis and viability of the yeast Saccharomyces cerevisiae. FEMS Microbiol. Lett. 2004;238(1):241-248.
Korcová J, Machová E, Filip J, Bystrický S. Biophysical properties of carboxymethyl derivatives of mannan and dextran. Carbohydr. Polym. 2015;134:6-11.
Korolenko TA, Kisarova YA, Filjushina EE, Dergunova MA, Machova E. Macrophage Stimulation and β-D-Glucans as Biological Response Modifi ers: The Role in Experimental Tumor Development. Handbook of Macrophages: Life Cycle, Functions and Diseases. Takahashi R, Kan H, eds. New York, 2012. P. 249-276.
Korolenko TA, Rukavishnikova EV, Safi na AF, Dushkin MI, Mynkina GI. Endocytosis by liver cells during suppression of intralysosomal proteolysis. Biol. Chem. Hoppe Seyler. 1992;373(7):573-580.
Korolenko TA, Svechnikova IG, Filjushina EE, Kaledin VI, Vakulin GM, Usynin IF, Tsyrendordjiev DD. Macrophage stimulation and antitumor effect of Ukrain. Drugs Exp. Clin. Res. 1998;24(5-6):253-260.
Le Guezennec X, Brichkina A, Huang YF, Kostromina E, Han W, Bulavin DV. Wip1-dependent regulation of autophagy, obesity, and atherosclerosis. Cell Metab. 2012;16;(1):68-80.
Libjaková L, Bystrický S, Lizicárová I, Paulovicová E, Machová E. Evaluation of different mannan polysaccharide usage in enzyme-linked immunosorbent assay for specifi c antibodies determination. J. Pharm. Biomed. Anal. 2007;45(3):521-525.
Martino F, Puddu PE, Pannarale G, Colantoni C, Martino E, Niglio T, Zanoni C, Barillà F. Low dose chromium-polynicotinate or policosanol is effective in hypercholesterolemic children only in combination with glucomannan. Atherosclerosis. 2013;228(1):198-202.
Nguyen DG, Hildreth JE. Involvement of macrophage mannose receptor in the binding and transmission of HIV by macrophages. Eur. J. Immunol. 2003;33(2):483-493.
Pągowska-Klimek I, Cedzyński M. Mannan-binding lectin in cardiovascular disease. Biomed. Res. Int. 2014;2014. ID 616817. doi: 10.1155/2014/616817.
Pisareva EE, Goncharova IA, Tuzikov FV, Goncharova NV, Machova E, Korolenko TA. Role of changes in serum chitotriosidase activity in mice under conditions of hyperlipidemia and lipid-lowering effect of carboxymethylated (1-3)-β-Dglycan. Bull. Exp. Biol. Med. 2014;157(5):555-559.
Shibata N, Suzuki A, Kobayashi H, Okawa Y. Chemical structure of the cell-wall mannan of Candida albicans serotype A and its difference in yeast and hyphal forms. Biochem. J. 2007;404(3):365-372.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 7, pp. 24-28, July, 2016
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Goncharova, N.V., Khrapova, M.V., Pupyshev, A.B. et al. Hypolipidemic Effect of Mannan in Mice with Acute Lipemia Induced by Poloxamer 407. Bull Exp Biol Med 162, 18–22 (2016). https://doi.org/10.1007/s10517-016-3534-8
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DOI: https://doi.org/10.1007/s10517-016-3534-8