Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alliluev AP, Kotel’nikova OV, Kuvakina VI, Bobyleva GV, Gofman IL, Kaz’mina YuG. The relationship between immunological effectiveness of dry polysaccharide meningococcus group A vaccine and molecular parameters of group A polysaccharide. Zh. Mikrobiool. Epidemiol. Immunol. 1995;(4):67-71. Russian.
Kotel’nikova OV, Alliluev AP, Drozhzhina EYu, Koroleva IS, Sitnikova EA, Zinchenko AA, Gordeeva EA, Melikhova TD, Nokel EA, Zhigis LS, Zueva VS, Razgulyaeva OA, Serova OV, Yagudaeva EYu, Rumsh LD. Protective properties of recombinant igA1 protease from meningococcus. Biomed. Khim. 2014;60(4):479-486. Russian.
Kotel’nikova OV, Zinchenko AA, Gordeeva EA, Melikhova TD, Nokel EA, Zhigis LS, Zueva VS, Drozhzhina EYu, Serova OV, Rumsh LD. Prospects in useing proteases secreted by the microbe for the prevention of meningococcal meningitis. Clinical Medicine. Moscow, 2015. pp. 17-26. Russian.
Kuznetsova SA, Kositikiya LS, Sokolov D, Freidlin IS, Polosukhina ER, Baryshnikov AYu. Identification of an inducible intercellular adhesion molecule i expression on human endothelial cell UNE ECV304, using a cell enzyme-linked immunosorbent assay. Med Immunol. 1999;1(5):71-74. Russian.
Orlov AV, Burenin AG, Shipunova VO, Lizunova AA, Gorshkov BG, Nikitin PI. Development of Immunoassays Using Interferometric Real-Time Registration of Their Kinetics. Acta Naturae. 2014;6(1):85-95.
Rumsh LD, Mel’nikov EE, Alliluev AP, Kozlov LV, Kotel’nikova OV, Zhigis LS, Serova OV, Jagudaeva EJ, Bichucher AM, Zubov VP, Anokhina IV, Zueva VS, Avakov AE. Patent RF No. 2453599. Nucleic acid coding functionally active recombinant IgAL protease Neisseria meningitidis of serogroup B, recombinant plasmid DNA containing nucleotide sequence coding active IgAL protease, producer strain containing plasmid DNA producing mature form of IgAL protease, recombinant Ig protease Neisseria meningitidis of serogroup B, method for producing mature form IgAL protease showing immunogenic and protective properties. Bull. No. 17 (June 20, 2012).
Rumsh LD, Serova OV, Zinchenko AA, Alliluev AP, Kozlov LV, Kotel’nikova OV, Zhigis LS, Jagudaeva EJ, Andina SS, Anokhina IV, Zueva VS, Gordeeva EA, Melikhova TD, Nokel’ EA. Patent RF No. 2486243. Polynucleotide encoding mutant recombinant IgA1 protease of Neisseria meningitidis of serogroup B, recombinant plasmid DNA comprising said polynucleotide, host cell containing said plasmid DNA, recombinant IgA1 protease of Neisseria memingitidis of serogroup B, method of producing mature form of IgA1 protease. Bull. No. 18 (June 27, 2013).
Serova OV, Melnikov EE, Zinchenko AA, Kotel’nikova OV, Gordeeva EA, Zhigis LS, Zueva VS, Melikhova TD, Nokel EA, Yagudaeva EY, Rumsh LD, Bichucher AM, Kozlov LV, Alliluev AP. Recombinant IGA1 protease from N. meningitidis. Obtaining and properties. Biofarm. Zh. 2011;3(6):42-47. Russian.
Chibiskova OV, Kotel’nikova OV, Nesmeyanov VA, Alliluev AP, Vol’pina OM, Koroev DO, Filatova MP. Mechanisms of defense formation against meningococcal infection in mice immunized with synthetic peptides. Bull. Exp. Biol. Med. 2007;143(6):720-722.
Yagudaeva EYu, Zhigis LS, Razgulyaeva OA, Zueva VS, Melnikov EE, Zubov VP, Kotel’nikova OV, Rumsh LD, Kozlov LV, Bichucher AM, Alliluev AP, Avakov AE. Isolation and determination of the activity of IgA1 protease from Neisseria meningitidis. Russ. J. Bioorgan. Chem. 2010;36(1):81-89.
Henderson IR, Nataro JP. Virulence functions of autotransporter proteins. Infect. Immun. 2001;69(3):1231-1243.
Kazeeva TN, Shevelev AB. Unknown functions of immunoglobulins A. Biochemistry (Mosc.). 2007; 72(5):485-494.
Mistry D, Stockley RA. IgA1 protease. Int. J. Biochem. Cell Biol. 2006; 38(8):1244-1248.
Plaut AG, Bachovchin WW. IgA-specific prolyl endopeptidases: Serine type. Methods in Enzymology. New York, 1994. Vol. 244. pp. 137-151.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 3, pp. 369-372, March, 2016
Rights and permissions
About this article
Cite this article
Kotelnikova, O.V., Zinchenko, A.A., Vikhrov, A.A. et al. Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins. Bull Exp Biol Med 161, 391–394 (2016). https://doi.org/10.1007/s10517-016-3422-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3422-2