The effect peptides KE, KED, AED and AEDG on proliferation (Ki-67), regeneration and aging (CD98hc), apoptosis (caspase-3), and extracellular matrix remodeling (MMP-9) in skin fibroblasts during their aging in culture were studied by immunofluorescent confocal microscopy. All studied peptides inhibited MMP-9 synthesis that increases during aging of skin fibroblasts and enhanced the expression of Ki-67 and CD98hc that are less intensively synthesized during cell aging. Peptides AED and AEDG suppressed caspase-dependent apoptosis that increases during aging of cell cultures.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
O. V. Belova, V. Ya. Arion, and V. I. Sergienko, The role of cytokines in immune function of the skin, Immunopatol., Allergol., Infektol., No. 1, 41-55 (2008).
G. A. Ryzhak, T. N. Korol’kova, and E. V. Voiton, Gerontological cosmetology: prevention and correction of age-related changed in the skin [in Russian], St. Petersburg (2006).
V. Kh. Khavinson and G. A. Ryzhak, Peptide bioregulators in correction of age-related changes. Estet. Med., 9, No. 4, 409-413 (2010).
V. Kh. Khavinson, S. I. Tarnovskaya, N. S. Linkova, E. O. Gutop, and E. V. Elashkina, Epigenetic aspects of peptidergic regulation of vascular endothelial cell proliferation during aging. Uspekhi Gerontol., 27, No. 1, 108-114 (2014).
N. I. Chalisova, N. S. Linkova, A. N. Zhekalov, A. O. Orlova, G. A. Ryzhak, and V. Kh. Khavinson, Short peptides stimulate skin cell regeneration during ageing. Uspekhi Gerontol., 27, No. 4, 699-703 (2014).
V. N. Anisimov and V. K. Khavinson, Peptide bioregulation of aging: results and prospects. Biogerontology, 11, No. 2, 139-149 (2010).
E. Boulter, S. Estrach, A. Errante, C. Pons, L. Cailleteau, F. Tissot, G. Meneguzzi, and C. C. Feral, CD98hc (SLC3A2) regulation of skin homeostasis wanes with age. J. Exp. Med., 210, No. 1, 173-190 (2013).
T. L. Fernandez, D. R. Van Lonkhuyzen, R. A. Dawson, M. G. Kimlin, and Z. Upton, In vitro investigations on the effect of dermal fibroblasts on keratinocyte responses to ultraviolet B radiation. Photochem. Photobiol., 90, No. 6, 1332-1339 (2014).
C. A. Ghisalberti, A. Morisetti, A. Bestetti, and G. Cairo, Potent trophic activity of spermidine supramolecular complexes in in vitro models. World J. Biol. Chem., 4, No. 3, 71-78 (2013).
A. C. Jahns, H. Eilers, R. Ganceviciene, and O. A. Alexeyev Propionibacterium species and follicular keratinocyte activation in acneic and normal skin. Br. J. Dermatol., 172, No. 4, 981-987 (2015).
Z. Ulakcsai, F. Bagaméry, I. Vincze, E. Szoko, and T. Tabi, Protective effect of resveratrol against caspase 3 activation in primary mouse fibroblasts. Croat. Med. J., 56, No. 2, 78-84 (2015).
X. W. Wang, Y. Yu, and L. Gu, Dehydroabietic acid reverses TNF-a-induced the activation of FOXO1 and suppression of TGF-1/Smad signaling in human adult dermal fibroblasts. Int. J. Clin. Exp. Pathol., 7, No. 12, 8616-8626 (2014).
V. W. Wong, R. K. Garg, M. Sorkin, K. C. Rustad, S. Akaishi, K. Levi, E. R. Nelson, M. Tran, R. Rennert, W. Liu, M. T. Longaker, R. H. Dauskardt, and G. C. Gurtner, Loss of keratinocyte focal adhesion kinase stimulates dermal proteolysis through upregulation of MMP9 in wound healing. Ann. Surg., 260, No. 6, 1138-1146 (2014).
S. N. Xue, J. Lei, D. Z. Lin, C. Yang, and L. Yan, Changes in biological behaviors of rat dermal fibroblasts induced by high expression of MMP9. World J. Emerg. Med., 5, No. 2, 139-143 (2014).
P. Zhang, J. Li, X. Tang, J. Zhang, J. Liang, and G. Zeng, Dracorhodin perchlorate induces apoptosis in primary fibroblasts from human skin hypertrophic scars via participation of caspase-3. Eur. J. Pharmacol., 728, 82-92 (2014).
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 40-44, January, 2016
About this article
Cite this article
Lin’kova, N.S., Drobintseva, A.O., Orlova, O.A. et al. Peptide Regulation of Skin Fibroblast Functions during Their Aging In Vitro . Bull Exp Biol Med 161, 175–178 (2016). https://doi.org/10.1007/s10517-016-3370-x