We studied the effects of cooling to -10°C and re-warming to 37°C on slices of rat olfactory cortex. The amplitudes of action potential in the lateral olfactory tract and excitatory postsynaptic potential activated by AMPA recovered during slow cooling/re-warming (0.1°C/min), while during rapid cooling/re-warming (9°C/min), they surpassed the control values. NMDA receptor-dependent mechanism was blocked in both cooling/re-warming modes. Swelling of the brain slices was observed during re-warming, especially during rapid cooling/re-warming. Nerve fibers of the lateral olfactory tract and AMPA-related processes survived deep cooling/re-warming, while NMDA-related processes were irreversibly blocked.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 1, pp. 36-39, January, 2016
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Mokrushin, A.A. Effects of Deep Cooling and Re-Warming on Ionotropic Glutamatergic Receptors In Vitro . Bull Exp Biol Med 161, 28–31 (2016). https://doi.org/10.1007/s10517-016-3337-y
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DOI: https://doi.org/10.1007/s10517-016-3337-y