The effects of liposomes containing phospholipid cardiolipin without antibiotic and loaded with levofloxacin on the growth of Mycobacterium tuberculosis with extensive drug resistance were studied in vitro. Liposomes consisting of cardiolipin alone in a concentration of 335 μM completely suppressed the growth of M. tuberculosis. In order to reduce the minimum inhibitory concentration of cardiolipin, complex liposome preparation consisting of phosphatidylcholin/cholesterol/cardiolipin and loaded with levofloxacin was prepared. Due to this, the cardiolipin concentration was reduced to 33.5 μM (50 μg/ml) and concentration of levofloxacin – to 2 μg/ml.
Similar content being viewed by others
References
S. N. Andreevskaya, T. G. Smirnova, Yu. A. Zhogina, et al., Dokl. Akad. Nauk, 434, No. 5, 705-708 (2010).
S. N. Andreevskaya, L. N. Chernousova, T. G. Smirnova, et al., Tuberkulez Bol. Legkikh, No. 12, 43-48 (2006)
Yu. L. Mikulovich, G. M. Sorokoumova, A. A. Selishcheva, and V. I. Shvets, Tonkie Khim. Tekhnol., 7, No. 4, 58-63 (2012).
S. V. Sidorenko, Rus. Med. Zh., 11, No. 2, 98-102 (2003).
T. G. Smirnova, Yu. L. Mikulovich, S. N. Andreevskaya, et al., Biofarm. Zh., 3, No. 2, 19-27 (2011).
G. M. Sorokoumova, S. N. Andreevskaya, T. G. Smirnova, et al., Bull. Exp. Biol. Med., 148, No. 5, 797-799 (2009).
G. M. Sorokoumova, Ya. O. Yasin, Yu. L. Mikulovich, et al., Tonkie Khim. Tekhnol., 8, No. 5, 72-76 (2013).
Tuberculosis in the Russian Federation, 2011. Analytical Review of Statistical Parameters Used in the Russian Federation and in the World [in Russian], Moscow (2013).
M. A. Kohanski, D. J. Dwyer, and J. J. Collins, Nat. Rev. Microbiol., 8, No. 6, 423-435 (2010).
C. H. Liu, L. Li, Z. Chen, et al., PLoS One, 6, No. 4, doi: 10.1371/journal.pone.0019399 (2011).
X. Liu, J. Wang, L. Miao, et al., Asian J. Pharm. Sci., 6, No. 6, 259-266 (2011).
T. Mizushima, S. Natori, and K. Sekimizu, Biochem. J., 285, Pt 2, 503-506 (1992).
V. A. Struchkov, N. B. Strazhevskaya, and R. I. Zhdanov, Bioelectrochemistry, 58, No. 1, 23-30 (2002).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 160, No. 11, pp. 626-630, November, 2015
Rights and permissions
About this article
Cite this article
Gaidukevich, S.K., Mikulovich, Y.L., Smirnova, T.G. et al. Antibacterial Effects of Liposomes Containing Phospholipid Cardiolipin and Fluoroquinolone Levofloxacin on Mycobacterium tuberculosis with Extensive Drug Resistance. Bull Exp Biol Med 160, 675–678 (2016). https://doi.org/10.1007/s10517-016-3247-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3247-z