We analyzed changes in cell composition of umbilical cord blood and functional activity of hematopoietic stem cells during cryogenic storage and after repeated freezing/thawing cycles. It was found that repeated freezing/thawing cycles performed according to the optimal programmable freezing protocol did not significantly affect viability and functional activity of hematopoietic stem cells. When fast freezing program was used, the cells completely lost their capacity to form colonies in semisolid medium, despite high viability parameters in the test with 7-AAD.
Similar content being viewed by others
References
H. Ali and H. Bahbahani, Acta Neurobiol. Exp. (Wars.), 70, No. 3, 316-324 (2010).
E. R. Andreeva, I. V. Andrianova, E. V. Sotnezova, et al., PLoS One, 10, No. 4, doi: 10.1371/journal.pone.0124939 (2015).
K. K. Ballen, E. Gluckman, and H. E. Broxmeyer, Blood, 122, No. 4, 491-498 (2013).
H. E. Broxmeyer, Semin. Hematol., 47, No. 1, 97-103 (2010).
P. Flores-Guzman, V. Fernandez-Sanchez, and H. Mayani, Stem Cells Transl. Med., 2, No. 11, 830-838 (2013).
M. Gunetti, I. Ferrero, D. Rustichelli, et al., Exp. Hematol., 36, No. 2, 235-243 (2008).
P. J. Hanley, C. M. Bollard, and C. G. Brunstein, Cytotherapy, 17, No. 6, 749-755 (2015).
E. V. Maslova, E. R. Andreeva, and I. V. Andrianova, Bull. Exp. Biol. Med., 156, No. 4, 584-589 (2014).
L. Metheny, P. Caimi, and M. de Lima, Front. Oncol., 3, 238 (2013).
C. Petrini, J. Biol. Med., 5, 87-97 (2014).
Yu. A. Romanov, E. E. Balashova, O. A. Bystrykh, et al., Bull. Exp. Biol. Med., 158, No. 4, 523-527 (2015).
Y. A. Romanov, O. P. Tarakanov, S. M. Radaev, et al., Cytotherapy, 17, No. 7, 969-978 (2015).
S. Roura, J. M. Pujal, C. Galvez-Monton, and A. Bayes-Genis, Stem Cell Res. Ther., 6, No. 1, 123 (2015).
J. Sun, J. Allison, C. McLaughlin, et al., Transfusion, 50, No. 9, 1980-1987 (2010).
J. M. Sun and J. Kurtzberg, Cytotherapy, 17, No. 6, 775-785 (2015).
F.Timeus, N. Crescenzio, P. Saracco, et al., Haematologica, 88, No. 1, 74-79 (2003).
A. E. Willing, S. N. Garbuzova-Davis, O. Zayko, et al., Cell Transplant., 23, No. 12, 1613-1630 (2014).
H. Yang, A. Pidgorna, M. R. Loutfy, and P. Shuen, Transfusion, 55, No. 1, 70-78 (2015).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 265-269, October, 2015
Rights and permissions
About this article
Cite this article
Romanov, Y.A., Balashova, E.E., Volgina, N.E. et al. Changes in Cell Composition of Umbilical Cord Blood and Functional Activity of Hematopoietic Stem Cells during Cryogenic Storage and Repeated Freezing/Thawing Cycles. Bull Exp Biol Med 160, 571–574 (2016). https://doi.org/10.1007/s10517-016-3220-x
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3220-x