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Therapy of Chronic Cardiosclerosis in WAG Rats Using Cultures of Cardiovascular Cells Enriched with Cardiac Stem Cell

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We developed a protocol for preparing cardiac cell culture from rat heart enriched with regional stem cells based on clonogenic properties and proliferation in culture in a medium with low serum content. Experiments on WAG rats with experimental ischemic myocardial damage showed that implantation of autologous regional stem cells into the left ventricle reduced the volume of cicatricial tissue, promoted angiogenesis in the damaged zone, and prevented the risk of heart failure development.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Moscow, Russia

    E. V. Chepeleva, S. V. Pavlova, A. A. Malakhova & S. M. Zakiyan

  2. Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Moscow, Russia

    E. V. Chepeleva, S. V. Pavlova, A. A. Malakhova & S. M. Zakiyan

  3. E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia

    E. V. Chepeleva, S. V. Pavlova, A. A. Malakhova, E. A. Milevskaya, Ya. L. Rusakova, N. A. Podkhvatilina, D. S. Sergeevichev, E. A. Pokushalov, A. M. Karaskov & S. M. Zakiyan

  4. Novosibirsk National Research State University, Novosibirsk, Russia

    E. A. Milevskaya & S. M. Zakiyan

  5. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    G. T. Sukhikh

Authors
  1. E. V. Chepeleva
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  2. S. V. Pavlova
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  3. A. A. Malakhova
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  4. E. A. Milevskaya
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  5. Ya. L. Rusakova
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  6. N. A. Podkhvatilina
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  7. D. S. Sergeevichev
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  8. E. A. Pokushalov
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  9. A. M. Karaskov
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  10. G. T. Sukhikh
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  11. S. M. Zakiyan
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Corresponding author

Correspondence to S. M. Zakiyan.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 191–200, July, 2015

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Chepeleva, E.V., Pavlova, S.V., Malakhova, A.A. et al. Therapy of Chronic Cardiosclerosis in WAG Rats Using Cultures of Cardiovascular Cells Enriched with Cardiac Stem Cell. Bull Exp Biol Med 160, 165–173 (2015). https://doi.org/10.1007/s10517-015-3119-y

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  • DOI: https://doi.org/10.1007/s10517-015-3119-y

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