Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 105 cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. Yu. Lupatov, A. S. Vdovin, I. V. Vakhrushev, et al., Bull. Exp. Biol. Med., Vol, No. 4, 220-227 (2014).
L. Lapasset, O. Milhavet, A. Prieur, et al., Genes Dev., 25, No. 21, 2248-2253 (2011).
N. Maherali and K. Hochedlinger, Cold Spring Harb. Symp. Quant. Biol., 73, 157-162 (2008).
K. Okita, M. Nakagawa, H. Hyenjong, et al., Science, 322, 949-953 (2008).
M. Stadtfeld, M. Nagaya, J. Utical, et al., Science, 322, 945-949 (2008).
K. Takahashi and S. Yamanaka, Cell, 126, No. 4, 663-676 (2006).
J. Yu, K. F. Chau, M. A. Vodyanik, et al., PLoS One, 6, No. 3, 10.1371/journal.pone.0017557 (2011).
Y. Zhao, X. Yin, H. Qin, et al., Cell Stem Cells, 3, No. 5, 475-479 (2008).
H. Zhou, S. Wu, J. Y. Joo, et al., Cell Stem Cells, 4, No. 5, 381-384 (2009).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Vdovin, A.S., Lupatov, A.Y., Kholodenko, I.V. et al. Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming. Bull Exp Biol Med 160, 123–128 (2015). https://doi.org/10.1007/s10517-015-3112-5
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-015-3112-5