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Morphological Criteria of Cell Differentiation Stages in Experimental Hepatocarcinoma and Evaluation of Antitumor Drug Efficiency

Bulletin of Experimental Biology and Medicine volume 160, pages 109–114 (2015)Cite this article

Structural polymorphism of 5 cell differentiation stages of hepatocarcinoma-29 from ascitic fluid is detected and the morphological criteria for identification of these stages are defined on the base of optic and electron microscopy findings, cytofluorometry, and DNA cytometry. The percentage of cells at differentiation stages 4 and 5 in the tumor structure increases after hepatocarcinoma cell inoculation into the hip. Injection of a cell cycle-modulating substance to animals with tumor growth shifts the proportion of cells with various differentiation stages. The morphological criteria of 5 stages of hepatocarcinoma-29 cell differentiation can be used for prospective drug testing.

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References

  1. N. P. Bgatova, Yu. I. Borodin, V. V. Makarova, et al., Bull. Exp. Biol. Med., 157, No. 1, 89-94 (2014).

    Article  CAS  PubMed  Google Scholar 

  2. T. S. Gerashchenko, E. V. Denisov, N. V. Litvyakov, et al., Biokhimiya, 78, No. 11, 1531-1549 (2013).

    Google Scholar 

  3. V. I. Kaledin, N. A. Zhukova, V. P. Nikolin, et al., Bull. Exp. Biol. Med., 148, No. 6, 903-907 (2010).

    Article  Google Scholar 

  4. E. V. Ovsjanko, A. V. Efremov, S. V. Michurina, et al., Bull. Exp. Biol. Med., 148, No. 3, 447-451 (2010).

    Article  Google Scholar 

  5. L. V. Omel’yachuk, V. F. Semeshin, A. L. Alekseeva, et al., Tsitologiya, 52, No. 4, 349-353 (2010).

    Google Scholar 

  6. I. N. Tyuryaeva, N. A. Filatova, Yu. M. Rozanov, et al., Tsitologiya, 52, No. 10, 817-826 (2010).

    Google Scholar 

  7. F. Colombo, F. Baldan, S. Mazzucchelli, et al., PLoS One, 6, No. 6, doi: 10.1371/journal.pone.0021369 (2011).

  8. R. A. Gatenby, A. S. Silva, R. J. Gillies, and B. R. Frieden, Cancer Res., 69, No. 11, 4894-4903 (2009).

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. A. P. Kusumbe and S. A. Bapat, Cancer Res., 69, No. 24, 9245-9253 (2009).

    Article  CAS  PubMed  Google Scholar 

  10. N. D. Marjanovic, R. A. Weinberg, and C. L. Chaffer, Clin. Chem., 59, No. 1, 168-179 (2013).

    Article  CAS  PubMed  Google Scholar 

  11. C. E. Meacham and S. J. Morrison, Nature, 501, 328-337 (2013).

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. E. M. Rasch, C. E. Lee, and G. A. Wyngaard, Genome, 47, No. 3, 559-564 (2004).

    Article  CAS  PubMed  Google Scholar 

  13. Y. Shen and D. Cao, Front. Biosci., 4E, No. 3, 1157-169 (2012).

    Article  Google Scholar 

  14. M. A. Walasek, L. Bystrykh, V. van den Bloom, et al., Blood, 119, No. 13, 3050-3059 (2012).

    Article  CAS  PubMed  Google Scholar 

  15. Q. Zhu, J. Yang, S. Han, et al., Prostate, 71, No. 8, 835-845 (2011).

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia

    N. P. Bgatova, A. A. Pozhidaeva, A. P. Lykov, O. V. Poveshchenko, O. P. Makarova, L. N. Rachkovskaya, Yu. I. Borodin & V. I. Konenkov

  2. Institute of Molecular and Cellular Biology, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    L. V. Omel’yanchuk & V. F. Semeshin

Authors
  1. N. P. Bgatova
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  2. L. V. Omel’yanchuk
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  3. A. A. Pozhidaeva
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  4. V. F. Semeshin
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  5. A. P. Lykov
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  6. O. V. Poveshchenko
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  7. O. P. Makarova
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  8. L. N. Rachkovskaya
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  9. Yu. I. Borodin
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  10. V. I. Konenkov
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Corresponding author

Correspondence to N. P. Bgatova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 160, No. 7, pp. 126-132, July, 2015

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Bgatova, N.P., Omel’yanchuk, L.V., Pozhidaeva, A.A. et al. Morphological Criteria of Cell Differentiation Stages in Experimental Hepatocarcinoma and Evaluation of Antitumor Drug Efficiency. Bull Exp Biol Med 160, 109–114 (2015). https://doi.org/10.1007/s10517-015-3110-7

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  • DOI: https://doi.org/10.1007/s10517-015-3110-7

Key Words

  • hepatocarcinoma-29
  • morphological criteria
  • differentiation stages