We present a simple and effective method for measuring urine S-adenosylhomocysteine by capillary electrophoresis without using modifiers. The detection threshold of the method is 0.1 μM S-adenosylhomocysteine, the time of analysis 13 min, reproducibility at physiological concentrations within 4%.
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References
A. V. Ivanov, B. P. Luzyanin, A. A. Moskovtsev, et al., Patol. Fiziol. Eksp. Ter., No. 2, 55-60 (2011).
B. P. Luzyanin, A. V. Ivanov, E. D. Viryus, et al., Patogenez, 12, No. 1, 15-20 (2014).
A. Becker, Y. M. Smulders, T. Teerlink, Eur. J. Clin. Invest., 33, No. 1, 17-25 (2003).
J. T. Brosnan, Can. J. Appl. Physiol., 29, No. 6, 773-780 (2004).
U. Delabar, D. Kloor, G. Luippold, and B. Mühlbauer, J. Chromatogr. B. Biomed. Sci. Appl., 724, No. 2, 231-238 (1999).
Z. Jiang, Q. Liang, G. Luo, et al., Talanta, 77, No. 4, 1279-1284 (2009).
D. M. Kerins, M. J. Koury, A. Capdevila, et al., Am. J. Clin. Nutr., 74, No. 6, 723-729 (2001).
J. Krijt, A. Duta, and V. Kozich, J. Chromatogr. B. Analyt. Technol. Biomed. Life Sc., 877, No. 22, 2061-2066 (2009).
S. C. Lu and J. M. Mato, Physiol. Rev., 92, No. 4, 1515-1542 (2012).
S. Melnyk, M. Pogribna, I. P. Pogribny, et al., Clin. Chem., 46, No. 2, 265-272 (2000).
R. Ramautar, G. W. Somsen, and G. J. de Jong, Electrophoresis, 30, No. 1, 276-291 (2009).
E. Sbrana, E. Bramanti, M. C. Spinetti, and G. Raspi, Electrophoresis, 25, Nos. 10-11, 1518-1521 (2004).
S. P. Stabler and R. H. Allen, Clin. Chem., 50, No. 2, 365-372 (2004).
E. O. Uthus, Electrophoresis, 24, No. 7-8, 1221-1226 (2003).
C. Wagner and M. J. Koury, Am. J. Clin. Nutr., 86, No. 6, 1581-1585 (2007).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 159, No. 4, pp. 525-528, April, 2015
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Luzyanin, B.P., Ivanov, A.V., Viryus, E.D. et al. S-Adenosylhomocysteine Assay in the Urine by Capillary Electrophoresis. Bull Exp Biol Med 159, 524–527 (2015). https://doi.org/10.1007/s10517-015-3008-4
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DOI: https://doi.org/10.1007/s10517-015-3008-4