At the early stages of mammalian embryo development, Ca2+ influences the processes of cleavage and differentiation. An important role in these processes is played by peptide growth factors. One of them, pentarphin, is a peptide growth factor similar to the central part of β-endorphin molecule and a fragment 369–373 of IgG heavy chain. We used fluorescent Ca2+ indicators Fluo-3 AM and Fura Red AM. Pentarphin added to embryos in a concentration of 0.1 μM 1.5-2 fold increased intercellular Ca2+ level in comparison with the initial level.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 159, No. 4, pp. 450–453, April, 2015
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Chernov, A.S., Sergievich, L.A. & Karnaukhova, N.A. Analysis of the Effect of β-Endorphin-Like Peptide Pentarphin on Intracellular Ca2+ Concentration in Early Mouse Embryos Using Fluorescent Probes. Bull Exp Biol Med 159, 459–462 (2015). https://doi.org/10.1007/s10517-015-2991-9
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DOI: https://doi.org/10.1007/s10517-015-2991-9