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Expression of HIF-1α in Multipotent Mesenchymal Stromal Cells under Hypoxic Conditions

  • BIOPHYSICS AND BIOCHEMISTRY
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Bulletin of Experimental Biology and Medicine Aims and scope

According to the data of reverse-transcription-PCR, long-term culturing of multipotent mesenchymal stromal cells from human adipose tissue at 1% O2 (vs. standard 20% O2) led to a decrease in HIF-1α gene expression, while no changes in this parameter were revealed at 5% O2. Analysis of the time course of HIF-1α mRNA level showed that at the early terms of hypoxic exposure, the expression of this gene increased and the duration of this increase depended on the severity of hypoxia.

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Authors and Affiliations

  1. State Scientific Center Institute of Biomedical Problems, Russian Academy of Medical Sciences, Moscow, Russia

    M. V. Pogodina & L. B. Buravkova

Authors
  1. M. V. Pogodina
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  2. L. B. Buravkova
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Correspondence to M. V. Pogodina.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 159, No. 3, pp. 333-335, March, 2015

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Pogodina, M.V., Buravkova, L.B. Expression of HIF-1α in Multipotent Mesenchymal Stromal Cells under Hypoxic Conditions. Bull Exp Biol Med 159, 355–357 (2015). https://doi.org/10.1007/s10517-015-2961-2

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  • DOI: https://doi.org/10.1007/s10517-015-2961-2

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