We proposed a new indicator for evaluation of functional activity of Ca2+ in human blood serum based on lysis of sheep erythrocytes with 10% human blood serum in the presence of 0.55 mM ethylene glycol tetraacetic acid at 37оC for 10 min. After incubation, the degree of sheep erythrocytes lysis inhibition is estimated: inhibition of complement hemolytic activity <30% is considered as high functional Са2+ activity, inhibition by 31-70% corresponds to normal activity, and >71% indicates low activity. The comparative studies of complement activating function of heterophilic antibodies, complement system reactivity in the presence of 0.29 M NaCl, and functional activity of ionized Ca2+ make possible estimation of the individual’s immune status.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 159, No. 3, pp. 281-284, March, 2015
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Shoibonov, B.B., Grigor’eva, D.V., Shabalina, A.A. et al. Estimation of Functional Activity of Ionized Calcium for Complement System Reactivity Assessment. Bull Exp Biol Med 159, 305–308 (2015). https://doi.org/10.1007/s10517-015-2948-z
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DOI: https://doi.org/10.1007/s10517-015-2948-z