Alloxan diabetes was modeled in August rats with high activity of the NO system and in Wistar rats, and the effects of NO system blockade (by a course treatment with L-NNA) on Langerhans islet β cells were studied in 15 days. The toxic effects of diabetes on the rat β cells and islets were similar: the content of active β cells in the islets decreased to 15-20%, the number of islets to 24-29% of control. A course of L-NNA reduced the β cell and islet death, in August cells greater than in Wistar: the number of islets in August rats was restored to 81%, in Wistar rats to 60% of initial level; the activity of β cells remained at the control level in the former and 2-fold lower than in the control in the latter. It seems that a less pronounced protective effect of L-NNA in Wistar rats was explained by excessive reduction of NO level essential for β cell regeneration.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 159, No. 2, pp. 238-243, February, 2015
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Smirnova, E.A., Michunskaya, A.B., Terekhina, O.L. et al. Effect of Nitric Oxide Synthesis Blockade on the Morphology of Langerhans Islets in August and Wistar Rats with Acute Alloxan Diabetes. Bull Exp Biol Med 159, 273–277 (2015). https://doi.org/10.1007/s10517-015-2940-7
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DOI: https://doi.org/10.1007/s10517-015-2940-7