Expression of 20 surface markers was analyzed in cultures of mesenchymal stromal cells of the umbilical cord, fibroblasts from adult and fetal human skin, and fi broblast-like cells of fetal liver was analyzed by fl ow cytometry. The studied cultures did not express hemopoietic cells markers, but were positive for CD73, CD90, and CD105 markers recommended by the International Society of Cell Therapy for the identifi cation of the multipotent mesenchymal stromal cells. Fetal liver fi broblast-like cells were positive for CD54; this marker was absent in skin fi broblast cultures, but was expressed by umbilical cord mesenchymal stromal cells. Further study of these cells revealed a minor subpopulation of cells co-expressing CD24 and CD90 or CD24 and CD54. We hypothesized that these cells probably participate in epithelial mesenchymal transition.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
P. A. Karalkin, N. E. Kovtun, A. Yu. Lupatov, et al., Bull. Exp. Biol. Med., 144, No. 6, 817–820 (2007).
A. Yu. Lupatov, P. A. Karalkin, Yu. G. Suzdal’tseva, et al., Bull. Exp. Biol. Med., 142, No. 4, 521–526 (2006).
Yu. V. Suzdal’tseva, V. V. Burunova, N. V. Petrakova, et al., Bull. Exp. Biol. Med., 143, No. 1, 147–154 (2007).
A. Bartholomew, C. Sturgeon, M. Siatskas, et al., Exp. Hematol., 30, No. 1, 42–48 (2002).
A. Blasi, C. Martino, L. Balducci, et al., Vasc. Cells, 3, No. 1, doi: 10.1186/2045-824X-3-5 (2011).
C. P. Chang, C. C. Chio, C. U. Cheong, et al., Clin. Sci. (Lond.), 124, No. 3, 165–176 (2013).
M. Dominici, K. Le Blanc, I. Mueller, et al., Cytotherapy, 8, No. 4, 315–317 (2006).
A. J. Friedenstein , R. K. Chailakhyan, N. V. Latsinik, et al., Transplantation, 17, No. 4, 331–340 (1974).
E. Fuchs and J. A. Segre, Cell, 100, No. 1, 143–155 (2000).
A. Harichandan and H. J. Buhring, Best Pract. Res. Clin. Haematol., 24, No. 1, 25–36 (2011).
S. C. Lim and S. H. Oh, Pathol. Res. Pract., 201, No. 7, 479–486 (2005).
M. Pereira, U. Pfi sterer, D. Rylander, et al., Sci. Rep., 4, doi: 10.1038/srep06330 (2014).
G. Petrof, M. Martinez-Queipo, J. E. Mellerio, et al., Br. J. Dermatol., 169, No. 5, 1025–1033 (2013).
M. F. Pittenger, A. M. Mackay, S. C. Beck, et al., Science, 284, 143–147 (1999).
J. Sanchez-Ramos, S. Song, F. Cardozo-Pelaez, et al., Exp. Neurol., 164, No. 2, 247–256 (2000).
G. Vogel, Science, 287, 1418–1419 (2000).
K. N. Yarygin, I. V. Kholodenko, R. T. Tairova, et al., J. Tissue Eng. Reg. Med., 6, Suppl. 1, 282 (2012).
H. Zhang, Z. Miao, Z. He, et al., Cell Biol. Int., 29, No. 3, 213–219 (2005).
Q. Zou, Q. Yan, J. Zhong, et al., J. Biol. Chem., 289, No. 8, 5250–5260 (2014).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 221–228, October, 2014
Rights and permissions
About this article
Cite this article
Lupatov, A.Y., Vdovin, A.S., Vakhrushev, I.V. et al. Comparative Analysis of the Expression of Surface Markers on Fibroblasts and Fibroblast-Like Cells Isolated from Different Human Tissues. Bull Exp Biol Med 158, 537–543 (2015). https://doi.org/10.1007/s10517-015-2803-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-015-2803-2