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Isolation and Culturing of Trophoblasts from Human Terminal Placenta

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Bulletin of Experimental Biology and Medicine Aims and scope

Trophoblast culture was derived from the islets of cell migrating from the fragments of placental microvilli. The isolated cells retained their cytotrophoblast phenotype longer (until passage 2–3) and then gained characteristics of proliferating mesenchymal cells. We conclude that changes in the morphology and co-expression of epithelial (cytokeratin 7) and mesenchymal (vimentin) markers attest to epithelial mesenchymal transition.

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References

  1. T. D. Kolokol’tsova and N. N. Saburina, Patogenez, No. 3, 29–31 (2013).

  2. G. Aboagye-Mathiesen, J. Laugesen, M. Zdravkovic, et al., Clin. Diagn. Lab. Immunol., 3, No. 1, 14–22 (1996).

    PubMed Central  CAS  PubMed  Google Scholar 

  3. F. Bellego, C. Vaillancourt, and J. Lafond, Methods Mol. Biol., 550, 73–87 (2009).

    Article  PubMed  Google Scholar 

  4. M. Cervac-Zivcovic and C. Stern, The Placenta: from Development to Disease, Eds. H. H. Kay, et al., Hoboken (2011), pp. 155–162.

  5. T. Ezashi, B. P. Telugu, and R. M. Roberts, Cell Tissue Res, 349, No. 3, 809–824 (2012).

    Article  PubMed Central  PubMed  Google Scholar 

  6. J. A. Irving, J. J. Lysiak, C. H. Graham, et al., Placenta, 16, No. 5, 413–433 (1995).

    Article  CAS  PubMed  Google Scholar 

  7. A. King, L. Thomas, and P. Bischof, Placenta, 21, Suppl. A, S113-S119 (2000).

    Article  PubMed  Google Scholar 

  8. H. J. Kliman, J. E. Nestler, E. Sermasi, et al., Endocrinology, 118, No. 4, 1567–1582 (1986).

    Article  CAS  PubMed  Google Scholar 

  9. M. Knofler and J. Pollheimer, Front. Genet., 4, 190 (2013).

    Article  PubMed Central  PubMed  Google Scholar 

  10. H. Logothetou-Rella, I. G. Kotoulas, J. M. Nesland, et al., Histol. Histopathol., 4, No. 3, 367–374 (1989).

    CAS  PubMed  Google Scholar 

  11. J. Maldonado-Estrada, E. Menu, P. Roques, et al., J. Immunol. Methods, 286, No. 1, 21–34 (2004).

    Article  CAS  PubMed  Google Scholar 

  12. K. Orendi, V. Kiviti, M. Sammar, et al., Placenta, 32, Suppl., S49-S54 (2011).

    Article  CAS  PubMed  Google Scholar 

  13. K. A. Pennington, J. M. Schlitt, and L. C. Schulz, J. Vis. Exp., 59, No. 59, e3202 (2012).

    PubMed  Google Scholar 

  14. M. G. Petroff, T. A. Phillips, H. Ka, et al., Methods Mol. Biol., 121, 203–217 (2006).

    Google Scholar 

  15. T. Takao, K. Assanoma, K. Kato, et al., PLoS One, 6, No. 7, doi: 10.1371/journal.pone.0021990 (2011).

  16. M. Zeisberg and E. G. Neilson, J. Clin. Invest., 119, No. 6, 1429–1437 (2009).

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia

    T. D. Kolokol’tsova, N. N. Saburina & G. T. Sukhikh

  2. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    P. A. Poltavtseva & G. T. Sukhikh

  3. Russian Medical Academy of Postgraduate Education, Moscow, Russia

    T. D. Kolokol’tsova, N. N. Saburina & G. T. Sukhikh

Authors
  1. T. D. Kolokol’tsova
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  2. N. N. Saburina
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  3. P. A. Poltavtseva
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  4. G. T. Sukhikh
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Corresponding author

Correspondence to T. D. Kolokol’tsova.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 216–220, October, 2014

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Kolokol’tsova, T.D., Saburina, N.N., Poltavtseva, P.A. et al. Isolation and Culturing of Trophoblasts from Human Terminal Placenta. Bull Exp Biol Med 158, 532–536 (2015). https://doi.org/10.1007/s10517-015-2802-3

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  • DOI: https://doi.org/10.1007/s10517-015-2802-3

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