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Umbilical Cord Blood for Autologous Transfusion in the Early Postnatal Ontogeny: Analysis of Cell Composition and Viability during Long-Term Culturing

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

An Erratum to this article was published on 19 March 2015

Changes in cell composition and viability as well as the content and functional activity of hemopoietic progenitor cells were analyzed during long-term (up to 1 month at 4°C) storage of human umbilical cord blood cells. No significant quantitative changes in erythrocytes were found during this period. The total content and viability of leukocytes changed, which resulted in the prevalence of mononuclear cells (lymphocytes and monocytes). Analysis of functional activity of hemopoietic stem cells in semisolid culture revealed a decrease in the relative content of CFU during the first week of culturing and inability of cells to colony formation after 2 weeks.

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Authors and Affiliations

  1. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    O. A. Bystrykh, K. V. Titkov, T. A. Fedorova, O. V. Rogachevskii, D. N. Degtyarev & G. T. Sukhikh

  2. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia

    Yu. A. Romanov, E. E. Balashova & N. V. Kabaeva

  3. CryoCenter Umbilical Cord Blood Bank, Moscow, Russia

    Yu. A. Romanov, E. E. Balashova & T. N. Dugina

Authors
  1. Yu. A. Romanov
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  2. E. E. Balashova
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  3. O. A. Bystrykh
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  4. K. V. Titkov
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  5. T. N. Dugina
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  6. N. V. Kabaeva
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  7. T. A. Fedorova
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  8. O. V. Rogachevskii
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  9. D. N. Degtyarev
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  10. G. T. Sukhikh
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Corresponding author

Correspondence to Yu. A. Romanov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 207-211, October, 2014

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Romanov, Y.A., Balashova, E.E., Bystrykh, O.A. et al. Umbilical Cord Blood for Autologous Transfusion in the Early Postnatal Ontogeny: Analysis of Cell Composition and Viability during Long-Term Culturing. Bull Exp Biol Med 158, 523–527 (2015). https://doi.org/10.1007/s10517-015-2800-5

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  • DOI: https://doi.org/10.1007/s10517-015-2800-5

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