Skip to main content
SpringerLink
Log in

Effects of Activated Protein C on the Size of Modeled Ischemic Focus and Morphometric Parameters of Neurons and Neuroglia in Its Perifocal Zone

  • MORPHOLOGY AND PATHOMORPHOLOGY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The effects of activated protein C (APC) on the quantitative parameters of neurons and neuroglia in the perifocal zone of infarction induced in the left hemispheric cortex were studied in two groups of rats. Group 1 animals served as control (control infarction). Group 2 rats were injected with APC (50 μg/kg) in the right lateral cerebral ventricle 3 h after infarction was induced, and after 72 h the infarction size was evaluated and the neurons and neuroglia in the perifocal zone were counted. APC reduced the infarction size 2.5 times in comparison with the control and reduced by 16% the neuronal death in the perifocal zone layer V, causing no appreciable changes in layer III, and did not change the size of neuronal bodies but increased (by 11%) the size of neuronal nuclei in layer III. The protein maintained the sharply increased count of gliocytes in the perifocal zone of infarction and promoted their growth. Hence, APC protected the neurons from death in the ischemic focus by increasing the gliocyte count and stimulating the compensatory reparative processes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. N. V. Vereshchagin, V. A. Morgunov, and T. S. Gulevskaya, Brain Pathology in Atherosclerosis and Arterial Hypertension [in Russian], Moscow (1997).

  2. S. A. Gavrilova, N. S. Samoilenkova, Yu. A. Pirogov, et al., Patogenez, 6, No. 3, 13-17 (2008).

    Google Scholar 

  3. Z. A. Suslina and Yu. Ya. Varakin, Ann. Klin. Eksp. Nevrol., No. 1, 22-28 (2007).

    Google Scholar 

  4. J. L. Cadet and I. N. Krasnova, Mol. Neurobiology, 39, No. 1, 50-61 (2009).

    Article  CAS  Google Scholar 

  5. A. S. Go, D. Mozaffarian, V. L. Roger, et al., Circulation, 127, No. 1, 143-152 (2013).

    Article  PubMed  Google Scholar 

  6. L. Gorbacheva, O. Davidova, E. Sokolova, et al., J. Neurochemistry, 111, 1471-1479 (2009).

    Article  Google Scholar 

  7. H. Guo, I. Singh, Y. Wang, et al., Eur. J. Neurosci., 29, No. 6, 1119-1130 (2009).

    Article  PubMed Central  PubMed  Google Scholar 

  8. K. A. Hossman, Cell. Mol. Neurobiol., 26, Nos. 7-8, 1057-1083 (2006).

    Google Scholar 

  9. U. Ito, T. Kuroiwa, J. Nagasao, et al., Stroke, 37, No. 8, 2134-2139 (2006).

    Article  PubMed  Google Scholar 

  10. K. Kuboyama, H. Harada, H. Tozaki-Saitohi, et al., J. Cereb. Blood Flow Metab., 31, No. 9, 1930-1941 (2011).

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. L. O. Mosnier, B. V. Zlokovic, and J. H. Griffin, Blood, 109, No. 8, 3161-3172 (2007).

    Article  CAS  PubMed  Google Scholar 

  12. T. P. Obrenovitch, Physiol. Rev., 88, No. 1, 211-247 (2008).

    Article  CAS  PubMed  Google Scholar 

  13. K. S. Panickar and M. D. Norenberg, Glia, 50, No. 4, 287-298 (2005).

    Article  PubMed  Google Scholar 

  14. A. Verkhratsky and A. Butt, Glial Neurobiology, Chichester (2007).

  15. B. V. Zlokovic, C. Zhang, D. Liu, et al., Ann. Neurol., 58, No. 3, 474-477 (2005).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Center of Neurology, Russian Academy of Medical Sciences, Moscow, Russia

    R. M. Khudoerkov, V. N. Sal’kov & V. B. Sobolev

  2. M. V. Lomonosov Moscow State University, Moscow, Russia

    I. G. Savinkova, S. M. Strukova, L. R. Gorbacheva, M. V. Gulyaev, Yu. A. Pirogov, S. A. Gavrilova & V. B. Koshelev

Authors
  1. R. M. Khudoerkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. G. Savinkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. M. Strukova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. R. Gorbacheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. V. Gulyaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yu. A. Pirogov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. V. N. Sal’kov
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. V. B. Sobolev
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. S. A. Gavrilova
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. V. B. Koshelev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. M. Khudoerkov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 157, No. 4, pp. 534-538, April, 2014

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Khudoerkov, R.M., Savinkova, I.G., Strukova, S.M. et al. Effects of Activated Protein C on the Size of Modeled Ischemic Focus and Morphometric Parameters of Neurons and Neuroglia in Its Perifocal Zone. Bull Exp Biol Med 157, 530–534 (2014). https://doi.org/10.1007/s10517-014-2607-9

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-014-2607-9

Keywords

Search

Navigation