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Measurement of Mitochondrial Membrane Potential in Leukocyte Suspension by Fluorescent Spectroscopy

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A method for evaluating mitochondrial membrane potential in isolated leukocyte suspension with the use of sensitive fl uorescent cation-active dye 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolocarbocyanine (JC-1) and spectrophotometry is described. JC-1 monomer rapidly penetrates through mitochondrial membrane of living cell and form aggregations characterized by red fl uorescence (λ = 585 nm). In case of mitochondrial membrane depolarization (early sign of apoptosis), JC-1 is not accumulated in the mitochondria and is present in the cytoplasm as a monomer characterized by green spectral fl uorescence (λ = 510 nm). The method can be used for evaluation of the function of living cells and mechanisms regulating energy metabolism by evaluating the mitochondrial membrane potential.

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Authors and Affiliations

  1. Research Institute of Medical Climatology and Rehabilitative Treatment, Vladivostok Affiliated Department of Far-Eastern Research Center of Respiration Physiology and Pathology, Siberian Division of the Russian Academy of Medical Sciences, Vladivostok, Russia

    E. G. Lobanova & E. V. Kondrat’eva

Authors
  1. E. G. Lobanova
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  2. E. V. Kondrat’eva
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Correspondence to E. V. Kondrat’eva.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 157, No. 2, pp. 258-260, February, 2014

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Lobanova, E.G., Kondrat’eva, E.V. Measurement of Mitochondrial Membrane Potential in Leukocyte Suspension by Fluorescent Spectroscopy. Bull Exp Biol Med 157, 288–290 (2014). https://doi.org/10.1007/s10517-014-2547-4

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  • DOI: https://doi.org/10.1007/s10517-014-2547-4

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