We studied the effect of blue light (440-490 nm) on the development of late blastocysts of mice carrying the gene of enhanced green fluorescent protein (EGFP). Exposure to blue light for 20 min reduced adhesive properties of blastocysts and their capacity to form primary colonies consisting of the cells of inner cell mass, trophoblast, and extraembryonic endoderm. The negative effects of blue light manifested in morphological changes in the primary colonies and impairment of differentiation and migration of cells of the trophoblast and extraembryonic endoderm. The problems of cell–cell interaction and inductive influences of the inner cell mass on other cell subpopulations are discussed. EGFP blastocysts were proposed as the model for evaluation of the mechanisms underlying the effects of blue light as the major negative factor of visible light used in in vitro experiments on mammalian embryos.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. N. Zubova, A. Yu. Bulavina, and A. P. Savitskii, Uspekhi Biol. Khimii, 43, 163-224 (2003).
L. M. Mezhevikina, V. V. Fedorova, I. V. Kapralova, and E. E. Fesenko, Ontogenez, 37, No. 1, 55-62 (2006).
N. Yu. Sakharova, E. F. Vikhlyantseva, A. A. Smirnov, and A. N. Konovalov, Ontogenez, 40, No. 5, 373-378 (2009).
A. A. Smirnov and E. F. Vikhlyantseva, Fundamental Science and Clinical Medicine [in Russian], St. Petersburg (2008), P. 334.
A. A. Smirnov, N. V. Shishova, N. Yu. Sakharova, et al., Bull. Exp. Biol. Med., 145, No. 1, 146-150 (2008).
B. P. Cormack, R. H. Valdivia, and S. Falkow, Gene, 173, No. 1, Spec. No., 33-38 (1996).
C. H. Hegele-Hartung, A. Schumacher, and B. Fisher, Anat. Embryol. (Berl.), 183, No. 6, 559-571 (1991).
M. Kato, K. Yamanouchi, M. Ikava, et al., Mol. Reprod. Dev., 54, No. 1, 43-48 (1999).
A. Nagy, M. Gertsenstein, K. Vintersten, and R. Behringer, Manipulating the Mouse Embryo: A Laboratory Manual. New York (2003).
S. B. Nicholls, J. Chu, G. Abbruzzese, et al., J. Biol. Chem., 286, No. 22, 24,977-24,981 (2011).
S. J. Oh, S. P. Gong, S. T. Lee, et al., Fertil. Steril., 88, No. 4, Suppl., 1150-1157 (2007).
N. Ouhibi, N. F. Sullivan, J. English, et al., Mol. Reprod. Dev., 40, No. 3, 311-324 (1995).
R. Shimizukawa, A. Sakata, M. Hirose, et al., Genesis, 42, No. 1, 47-52 (2005).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 52-56, January, 2014
Rights and permissions
About this article
Cite this article
Sakharova, N.Y., Mezhevikina, L.M., Smirnov, A.A. et al. Analysis of the Effects of Blue Light on Morphofunctional Status of In Vitro Cultured Blastocysts from Mice Carrying Gene of Enhanced Green Fluorescent Protein (EGFP). Bull Exp Biol Med 157, 162–166 (2014). https://doi.org/10.1007/s10517-014-2515-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-014-2515-z