The effects of Ridostin on the transcription of IFN family genes in human fibroblasts and lymphocytes were studied by quantitative real-time PCR. The degree of gene induction by Ridostin was most pronounced in fibroblasts, and was significantly higher than the induction by Kagocel: transcription of IFN-β, oligoadenylate synthetase, and double-stranded RNA-dependent protein kinase genes increased by about 2000, 100, and 20 times, respectively. In lymphocytes, Ridostin also activated a wide variety of IFN family genes, including genes of IFN-α, IFN-γ, and IFN-dependent enzymes, but this induction was less pronounced than in the fibroblasts. It was shown that gene response in lymphocyte from a child with cancer is reduced in comparison with that of adult healthy participant. Ridostin, and even more so Reaferon up-regulated activities of β-actin, glycerophosphate dehydrogenase, and β2- microglobulin genes, thus making impossible or limiting their use as constitutive stable reference genes (standards) in PCR-assays of IFN and their inductors.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 156, No. 8, pp. 179-182, August, 2013
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Sokolova, T.M., Shuvalov, A.N., Telkov, M.V. et al. Ridostin Induces Transcription of a Wide Spectrum of Interferon Genes in Human Cells. Bull Exp Biol Med 156, 213–216 (2013). https://doi.org/10.1007/s10517-013-2313-z
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DOI: https://doi.org/10.1007/s10517-013-2313-z