Argyrophilic proteins associated with chromosome nucleolar organizer regions (Ag-NOR proteins) were studied in lung squamous-cell carcinoma cells. After routine staining with silver nitrate, the area index and coefficient of variations of Ag-NOR protein were estimated by computer-aided image analysis. The Ag-NOR protein area indexes of tumor cells differed in clinical morphological variants of squamous-cell cancer (differing by T and N parameters, size of the primary node <3 cm or larger, by disease stage, and differentiation degree). Ag-NOR protein variation coefficient can be used for discriminating the tumors by size (<3 cm and larger), presence/absence of metastases, and evaluation of squamous-cell cancer differentiation degree. Ag-NOR protein area index and variation coefficient can be used as additional objective differential diagnostic criteria of clinical and morphological forms of squamous-cell carcinoma of the lung.
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References
I. P. Bobrov, A. M. Avdalyan, A. F. Lazarev, et al., Arkh. Patol., 70, No. 3, 18-23 (2008).
A. F. Lazarev, I. P. Bobrov, V. V. Klimachev, and V. A. Lubennikov, Arkh. Patol., 64, No. 6, 30-32 (2002).
A. F. Lazarev, D. S. Kobyakov, V. V. Klimachev, et al., Arkh. Patol., 72, No. 4, 16-20 (2010).
L. Antonangelo, F. del C. Bernardi, V. L. Capelozzi, et al., Chest, 111, No. 1, 110-114 (1997).
A. Bankfalvi, D. Ofner, K. W. Schmid, et al., Pathol. Res. Pract., 195, No. 4, 219-229 (1999).
V. Canet, M. P. Montmasson, Y. Usson, et al., Cytometry, 43, No. 2, 110-116 (2001).
M. Derenzini and D. Trere, J. Pathol., 165, 337-342 (1991).
R. S. Matheus, F. del C. Bernardi, C. P. Gallo, et al., Pathol. Res. Pract., 200, No. 1, 13-23 (2004).
A. Nonomura, Y. Mizukami, M. Oda, et al., Surg. Today, 23, No. 6, 486-490 (1993).
D. Ofner and K. W. Schmid, Histochem. Cell Biol., 106, No. 2, 193-196 (1996).
A. Pich, L. Chiusa, and E. Margaria, Micron, 31, No. 2, 133-141 (2000).
J. Piffko, A. Bankfalvi, D. Ofner, et al., J. Pathol., 182, No. 4, 450-456 (1997).
O. R. Rodriguez, L. Antonangelo, N. Yagi, et al., Jpn. J. Clin. Oncol., 27, No. 5, 298-304 (1997).
W. D. Travis, E. Brambilla, H. K. Muller-Hermelink, and C. C. Harris, World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of the Lung, Pleura, Thymus, and Heart, Lyon (2004).
D. Trere, Micron, 31, No. 2, 127-131 (2000).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 156, No. 7, pp. 93-97, July, 2013
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Kobyakov, D.S., Klimachev, V.V., Avdalyan, A.M. et al. Relationship between the Argyrophilic Proteins of the Nucleolar Organizer Region and Stage of Squamous-Cell Carcinoma of the Lung. Bull Exp Biol Med 156, 81–85 (2013). https://doi.org/10.1007/s10517-013-2283-1
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DOI: https://doi.org/10.1007/s10517-013-2283-1