Cytotoxic effect of doxorubicin in human multiple myeloma cell cultures RPMI 1640, RPMI 8226, and IM-9 was studied. The obtained data were compared with mRNA expression of MDR1, MRP1, BCRP, LRP genes responsible for the development of multiple drug resistance. IM-9 cells that differed from the other two cultures by the expression of surface differentiation markers and by mRNA expression of MDR1, BCRP, and LRP were most sensitive to doxorubicin. All cells expressed mRNA for only A-isoform of insulin receptor (IRA), while B-isoform (IRB) was not expressed. Insulin in a concentration of 5 μg/ml had no effect on the cytotoxic effect of doxorubicin in the studied cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
O. M. Votyakova and E. A. Demina, Multiple Myeloma. Clinical Oncohematology. Clinical Guidelines, Ed. M. A. Volkova [in Russian], Moscow (2007), pp. 847–873.
A. A. Stavrovskaya, Sorovskiy Obrazovat. Zhurn., 7, No. 7, 17–23 (2001).
A. A. Stavrovskaya, Biol. Membrany, 20, No. 3, 196–205 (2003).
S. S. Shushanov, Bull. Exp. Biol. Med., 150, No. 1, 42–44 (2010).
S. S. Shushanov, Bull. Exp. Biol. Med., 152, No. 2, 202–205 (2011).
S. S. Shushanov, L. G. Mar’ina, Yu. B. Chernykh, et al., Klin. Onkogematol., 3, No. 2, 105–113 (2010).
K. C. Anderson, R. A. Kyle, W. S. Dalton, et al., Hematology, 2000, No. 1, 147–165 (2000).
A. Dispenzieri and R. A. Kyle, Best Pract. Res. Clin. Haematol., 18, No. 4, 553–568 (2005).
G. G. Freund, D. T. Kulas, and R. A. Mooney, J. Immunol., 151, No. 4, 1811–1820 (1993).
J. Hatok, P. Račay, J. Hudeček, and D. Dobrota, Biologia, Bratislava, 61, No. 3, 247–256 (2006).
L. A. Hazlehurst, J. S. Damiano, I. Buyuksal, et al., Oncogene, 19, No. 3, 4319–4327 (2000).
T. Hideshima, P. L. Bergsagel, W. M. Kuehl, and K. C. Anderson, Blood, 104, No. 38, 607–618 (2004).
N. Kalitin, M. Kostjukova, E. Kakpakova, et al., Eur. J. Cancer, 47, No. 1, S644 (2011).
P. Sonneveld, J. Intern. Med., 247, No. 5, 521–534 (2000).
A. C. Sprynski, D. Hose, A. Kassambara, et al., Leukemia, 24, No. 11, 1940–1950 (2010).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 155, No. 2, pp. 195-200, February, 2013
Rights and permissions
About this article
Cite this article
Shushanov, S.S., Kravtsova, T.A. Cytotoxic Effect of Doxorubicin on Human Multiple Myeloma Cells In Vitro . Bull Exp Biol Med 155, 228–232 (2013). https://doi.org/10.1007/s10517-013-2120-6
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-013-2120-6