Various calcium phosphate ceramic materials were created and their effect on cultured mesenchymal cells from exfoliated deciduous tooth pulp was evaluated. Tricalcium phosphate ceramics provides best cell survival and is an optimal material for bone tissue engineering. Analysis of the effects of tricalcium phosphate ceramics on osteogenic differentiation of SHED cells suggests that this material potentiated dexamethasone-induced osteogenic differentiation, which manifested in the increased number of ossification foci and enhanced extracellular matrix production by cells. Thus, the tricalcium phosphate ceramics created by us is a promising biomedical material that can be used for tissue-engineered bone analogs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
E. N. Antonov, A. S. Bochkova, and A. V. Popova, Supercritical Fluids: Theory and Practice [in Russian], 6, No. 4, 13-20 (2011).
I. V. Vakhrushev, E. N. Antonov, A. V. Popova, et al., Kletoch. Tekhnol. Biol. Med., No. 1, 29-33 (2012); Springer: Bull. Exp. Biol. Ved., 153, No. 1, 143-147 (2012).
I. V. Vakhrushev, Yu. V. Suzdal’tseva, V. V. Burunova, et al., Kletoch. Tekhnol. Biol. Med., No. 1, 55-60 (2010); Springer: Bull. Exp. Biol. Ved., 149, No. 1, 161-165 (2010).
E. N. Antonov, V. N. Bagratashvili, M. J. Whitaker, et al., Adv. Mater. Deerfi eld., 17, No. 3, 327-330 (2004).
K. Chadipiralla, J. M. Yochim, B. Bahuleyan, et al., Cell. Tissue Res., 340, No. 2, 323-333 (2010).
M. Chimutengwende-Gordon and W. S. Khan, Curr. Stem Cell Res. Ther., 7, No. 2, 122-126 (2012).
Z. Gamie, G. T. Tran, G. Vyzas, et al., Expert. Opin. Biol. Ther., 12, No. 6, 713-729 (2012).
R. Z. LeGeros, Chem. Rev., 108, No. 11, 4742-4753 (2008).
M. Miura, S. Gronthos, M. Zhao, et al., Proc. Natl. Acad. Sci. USA., 100, No. 10, 5807-5812 (2003).
A. Polini, D. Pisignano, M. Parodi, et al., PLoS One, 6, No. 10, e26211 (2011).
B. M. Seo, W. Sonoyama, T. Yamaza et al., Oral Dis., 14, No. 5, 428-434 (2008).
Y. Zheng, Y. Liu, C. M. Zhang, et al., J. Dent. Res., 88, No. 3, 249-254 (2009).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 21-27, January, 2013
Rights and permissions
About this article
Cite this article
Vakhrushev, I.V., Smirnov, V.V., Goldberg, M.A. et al. Effect of Calcium Phosphate Materials on Multipotent Mesenchymal Cells from Exfoliated Deciduous Teeth (SHED Cells) in Vitro . Bull Exp Biol Med 155, 139–144 (2013). https://doi.org/10.1007/s10517-013-2099-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-013-2099-z