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Effect of Calcium Phosphate Materials on Multipotent Mesenchymal Cells from Exfoliated Deciduous Teeth (SHED Cells) in Vitro

Bulletin of Experimental Biology and Medicine volume 155, pages 139–144 (2013)Cite this article

Various calcium phosphate ceramic materials were created and their effect on cultured mesenchymal cells from exfoliated deciduous tooth pulp was evaluated. Tricalcium phosphate ceramics provides best cell survival and is an optimal material for bone tissue engineering. Analysis of the effects of tricalcium phosphate ceramics on osteogenic differentiation of SHED cells suggests that this material potentiated dexamethasone-induced osteogenic differentiation, which manifested in the increased number of ossification foci and enhanced extracellular matrix production by cells. Thus, the tricalcium phosphate ceramics created by us is a promising biomedical material that can be used for tissue-engineered bone analogs.

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Affiliations

  1. Stemma Research-and-Production Complex, Moscow, Russia

    I. V. Vakhrushev & K. N. Yarygin

  2. V. N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia

    I. V. Vakhrushev, P. A. Karalkin, A. Yu. Lupatov & K. N. Yarygin

  3. A. A. Baikov Institute of Metallurgy and Material Science, Russian Academy of Sciences, Moscow, Russia

    V. V. Smirnov, M. A. Goldberg & S. M. Barinov

Authors
  1. I. V. Vakhrushev
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  2. V. V. Smirnov
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  3. M. A. Goldberg
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  4. P. A. Karalkin
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  5. A. Yu. Lupatov
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  6. S. M. Barinov
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  7. K. N. Yarygin
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Corresponding author

Correspondence to I. V. Vakhrushev.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 21-27, January, 2013

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Vakhrushev, I.V., Smirnov, V.V., Goldberg, M.A. et al. Effect of Calcium Phosphate Materials on Multipotent Mesenchymal Cells from Exfoliated Deciduous Teeth (SHED Cells) in Vitro . Bull Exp Biol Med 155, 139–144 (2013). https://doi.org/10.1007/s10517-013-2099-z

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  • DOI: https://doi.org/10.1007/s10517-013-2099-z

Key Words

  • mesenchymal stem cells
  • dental pulp
  • calcium phosphates
  • tricalcium phosphate
  • tissue engineering