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Preparation of Fab-Fragments of GD2-Specific Antibodies and Analysis of Their Antitumor Activity In Vitro

  • Oncology
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Bulletin of Experimental Biology and Medicine Aims and scope

Monoclonal antibodies ME361 specific to ganglioside GD2 were isolated from the conditioned medium of hybridoma HB9326 and mouse ascitic fluid by the method of affinity chromatography; their Fab-fragments were obtained by proteolytic cleavage with papain. Evaluation of Fab-fragment specificity by flow cytometry and dot-blot analysis showed that binding effectiveness of fragments with antigens was close to that for the full-length molecule of antigen. It was shown that Fab-fragments and whole antibodies ME361 dose-dependently inhibit the proliferation of cells of mice T-lymphoma EL-4, and induce apoptosis of these cells 24 h after incubation.

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Authors and Affiliations

  1. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia

    I. I. Doronin, I. M. Molotkovskaya & R. V. Kholodenko

  2. Institute of General Pathology and Pathophysiology, Russian Academy of Medical Science, Moscow, Russia

    I. V. Kholodenko

Authors
  1. I. I. Doronin
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  2. I. V. Kholodenko
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  3. I. M. Molotkovskaya
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  4. R. V. Kholodenko
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Correspondence to I. V. Kholodenko.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 154, No. 11, pp. 616–622, November, 2012

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Doronin, I.I., Kholodenko, I.V., Molotkovskaya, I.M. et al. Preparation of Fab-Fragments of GD2-Specific Antibodies and Analysis of Their Antitumor Activity In Vitro . Bull Exp Biol Med 154, 658–663 (2013). https://doi.org/10.1007/s10517-013-2024-5

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  • DOI: https://doi.org/10.1007/s10517-013-2024-5

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