Organotypic culture was used to study the effects of glycoside ouabain and its aglycone ouabagenin on the growth of skin explants from 10-12-day chicken embryos. The tested agents demonstrated dose-dependent inhibition of skin growth. The effective concentrations imply that ouabain and ouabagenin mediate their inhibitory effect on the skin growth via interaction with α1-isoform of Na+,K+-ATPase that plays mostly the role of ionic pump.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 154, No. 10, pp. 410-411, October, 2012
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Penniyaynen, V.A., Kipenko, A.V., Lopatina, E.V. et al. Effect of Ouabain on Growth of Skin Explants in Organotypic Culture. Bull Exp Biol Med 154, 419–420 (2013). https://doi.org/10.1007/s10517-013-1965-z
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DOI: https://doi.org/10.1007/s10517-013-1965-z