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Preparation of Recombinant Atoxic Form of Exotoxin A from Pseudomonas Aeruginosa

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Nucleotide sequences encoding full-length protein of Pseudomonas aeruginosa exotoxin A and its atoxic form were cloned and expressed in Escherichia coli cells. Purified recombinant exotoxin and immune rabbit sera protected mice from exotoxin A.

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Authors and Affiliations

  1. I. I. Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia

    A. A. Kaloshin, M. A. Isakov, N. A. Mikhailova & Ju. V. Vertiev

Authors
  1. A. A. Kaloshin
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  2. M. A. Isakov
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  3. N. A. Mikhailova
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  4. Ju. V. Vertiev
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Corresponding author

Correspondence to A. A. Kaloshin.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 154, No. 9, pp. 330-335, September, 2012

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Kaloshin, A.A., Isakov, M.A., Mikhailova, N.A. et al. Preparation of Recombinant Atoxic Form of Exotoxin A from Pseudomonas Aeruginosa . Bull Exp Biol Med 154, 346–350 (2013). https://doi.org/10.1007/s10517-013-1947-1

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  • DOI: https://doi.org/10.1007/s10517-013-1947-1

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